ABCC11 Antibody (4H6) recommended for detection of ABCC11 of human origin by WB, IP, IF and ELISA

ABCC11 Antibody (4H6): sc-517150

ABCC11 Antibody (4H6) is rated 4.0 out of 5 by 1.
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Datasheets

    • Anti-ABCC11 Antibody (4H6) is a mouse monoclonal IgG2a (kappa light chain) ABCC11 antibody provided at 100 µg/ml
    • raised against amino acids 433-532 representing partial length ABCC11 of human origin
    • recommended for detection of ABCC11 of human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

I am using ABCC11 (4H6): sc-517150 for immunofluorescence, what dilution of this antibody should I use?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24

hi how many western blot test can be done using ABCC11 Antibody (4H6): sc-517150? thanks

Asked by: mustafa
Thank you for your question. This antibody, ABCC11 (4H6): sc-517150, is a mouse monoclonal IgG2a provided at 100 µg in 1 ml. We recommend testing for optimal dilution within a range of 1:100-1:1,000. If you were using the primary antibody at the most concentrated dilution of 1:100, in a final volume of 5 ml per membrane, then you could perform 20 Western blot tests. However, the number of tests is dependent upon what volume of buffer you use to incubate each membrane.
Answered by: Technical Support
Date published: 2016-12-01
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Rated 4 out of 5 by from good good antibody work well in WB at 1:200, bladder cancer
Date published: 2017-07-06
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