AAT Antibody (702): sc-66025
- AAT Antibody (702) is a mouse monoclonal IgG1 provided at 100 µg/ml
- raised against alpha-1-antitrypsin of human origin
- recommended for detection of AAT of human origin by WB and IP; non cross-reactive with alpha-1-antichymotrypsin (AACT)
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for AAT Antibody (702). This work is in progress.
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AAT Antibody (702) is a mouse monoclonal IgG1 antibody that detects α1-antitrypsin (AAT) in human samples through western blotting (WB) and immunoprecipitation (IP) applications. AAT plays a crucial role in protecting lung tissue from damage caused by Neutrophil Elastase, which is a significant factor in the development of pulmonary emphysema, a debilitating and irreversible lung disease characterized by the loss of lung elasticity. This 394 amino acid hepatic acute phase protein is primarily synthesized in the liver and is also found in cultured hepatoma cells and macrophages, highlighting AAT′s importance in both hepatic and immune responses. AAT is known for high polymorphism and glycosylation, which contribute to diverse isoelectric-focusing patterns across different variants. The gene encoding AAT is located on human chromosome 14, adjacent to a related serine protease inhibitor gene that encodes corticosteroid-binding globulin, indicating a complex regulatory network for protease inhibition. Notably, oxidation of the methionine 358 residue in AAT′s active site significantly impairs AAT′s ability to inhibit elastase, underscoring the importance of post-translational modifications in maintaining functional integrity. Furthermore, individuals with AAT deficiency face a 20-30 fold increased risk of developing early-onset pulmonary emphysema, making AAT study and interactions vital for understanding and potentially mitigating lung diseases.
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AAT Antibody (702) References:
- Laboratory diagnosis of alpha1-antitrypsin deficiency. | Ferrarotti, I., et al. 2007. Transl Res. 150: 267-74. PMID: 17964515
- The molecular and cellular pathology of α₁-antitrypsin deficiency. | Gooptu, B., et al. 2014. Trends Mol Med. 20: 116-27. PMID: 24374162
- A Library of Rare α1-Antitrypsin (AAT) Variant Phenotypes to Aid in the Diagnosis of AAT Deficiency. | Akbas, N., et al. 2016. Am J Clin Pathol. 146: 289-93. PMID: 27543976
- Association between α1-antitrypsin and acute coronary syndrome. | Liu, Y., et al. 2020. Exp Ther Med. 20: 119. PMID: 33005245
- Pregnancy in Alpha 1 Antitrypsin (AAT) Deficiency and the role of intravenous AAT therapy. | Guarnieri, G., et al. 2022. Pulmonology. 28: 317-319. PMID: 35221261
- Alpha1-antitrypsin deficiency and asthma. | Stirpe, E. and Bardaro, F. 2022. Monaldi Arch Chest Dis. 92: PMID: 35225443
- Protective α1-antitrypsin effects in autoimmune vasculitis are compromised by methionine oxidation. | Ebert, M., et al. 2022. J Clin Invest. 132: PMID: 36125911
- Safety of Intravenous Administration of an AAV8 Vector Coding for an Oxidation-Resistant Human α1-Antitrypsin for the Treatment of α1-Antitrypsin Deficiency. | Rosenberg, JB., et al. 2023. Hum Gene Ther. 34: 139-149. PMID: 36606685
- Computational Tools to Assist in Analyzing Effects of the SERPINA1 Gene Variation on Alpha-1 Antitrypsin (AAT). | Mróz, J., et al. 2024. Genes (Basel). 15: PMID: 38540399
- Analysis of alpha-1-antitrypsin (AAT)-regulated, glucocorticoid receptor-dependent genes in macrophages reveals a novel host defense function of AAT. | Bai, X., et al. 2024. Physiol Rep. 12: e16124. PMID: 39016119
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AAT Antibody (702) | sc-66025 | 100 µg/ml | $316.00 |