AAK1 Double Nickase Plasmid (h): sc-406073-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AAK1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- AAK1 Double Nickase Plasmid (h) and AAK1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AAK1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: AAK1 Antibody (LW-M11): sc-134242
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AAK1 Double Nickase Plasmid (h) | sc-406073-NIC | 20 µg | $410.00 | |||
AAK1 Double Nickase Plasmid (h2) | sc-406073-NIC-2 | 20 µg | $410.00 |
AAK1 (AP2-associated kinase 1) is a serine/threonine kinase that phosphorylates components of the AP2 adaptor complex to regulate clathrin-mediated endocytosis and membrane trafficking. By coordinating cargo internalization and receptor recycling, AAK1 influences signaling dynamics for pathways such as EGFR and GPCRs and shapes downstream cellular responses. It also interfaces with endosomal sorting and vesicle formation processes that impact nutrient uptake, synaptic vesicle cycling, and innate immune signaling. Altered AAK1 activity and endocytic control have been linked to dysregulated signaling and phenotypes relevant to cancer biology and neurological and infectious disease research contexts.
AAK1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AAK1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AAK1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AAK1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AAK1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.