A1BG Double Nickase Plasmid (h): sc-403087-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- A1BG Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- A1BG Double Nickase Plasmid (h) and A1BG Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting A1BG. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: A1BG Antibody (F-9): sc-374415
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
A1BG Double Nickase Plasmid (h) | sc-403087-NIC | 20 µg | $410.00 | |||
A1BG Double Nickase Plasmid (h2) | sc-403087-NIC-2 | 20 µg | $410.00 |
Alpha-1B-glycoprotein (A1BG) encodes a secreted plasma glycoprotein within the immunoglobulin superfamily that is primarily produced by liver and detected in circulation and extracellular fluids. Although its precise molecular function remains incompletely defined, A1BG is frequently studied in the context of extracellular protein interaction networks, glycoprotein turnover, and systemic inflammatory and metabolic states that influence the plasma proteome. Variation in A1BG abundance has been reported across multiple disease-associated proteomic profiles, supporting its use as a biomarker-linked target in translational discovery workflows. In cell and tissue models, interrogating A1BG can help connect secreted protein dynamics to pathways governing host response, tissue remodeling, and microenvironmental signaling.
A1BG Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the A1BG locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within A1BG. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt A1BG function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of A1BG-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.