A-673 + PMA nuclear extract: sc-2129
- supplied in four vials, each containing 250 µg nuclear extract in 50 µl buffer
- provided in 20 mM HEPES (pH 7.9), 20% v/v glycerol, 0.1 M KCI, 0.2 mM EDTA, 0.5 mM PMSF and 0.5 mM DTT
- human nuclear extract; rhabdomyosarcoma cells induced with phorbol
- suitable for use in Gel Shift and Western Blotting assays
- Extracts should be stored at -70°C and repeated freezing and thawing should be avoided.
- prepared by the method of Dignam et al., (1983) Nucleic Acids Res. 11: 1475
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SEE ALSO...
The A-673 + PMA nuclear extract is derived from the A-673 cell line, which originates from human sarcoma, a type of muscle cancer. This particular extract is treated with phorbol 12-myristate 13-acetate (PMA), a diester that is a known activator of protein kinase C (PKC). This activation can lead to a variety of cellular responses, particularly influencing gene expression and cellular differentiation pathways. The nuclear extract itself comprises a concentrated pool of nuclear proteins, including transcription factors, signaling intermediates, and chromatin-associated proteins that are responsive to PKC activation. In research, this nuclear extract is used to study the biochemical and molecular mechanisms by which PKC activation influences nuclear function and gene regulation, particularly in the context of cancer and cellular development. By employing the A-673 + PMA nuclear extract, researchers can explore how PKC-mediated pathways alter the nuclear landscape, potentially leading to changes in cellular behavior like proliferation, differentiation, and response to external signals. This understanding aids in dissecting complex signaling networks within cancerous cells, providing insights into the fundamental biology of tumor progression and the role of specific signaling cascades in cancer dynamics.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
A-673 + PMA nuclear extract | sc-2129 | 250 µg/0.05 ml | $160.00 |