A-10 nuclear extract: sc-24959

The A-10 nuclear extract is derived from the A-10 cell line, which is a smooth muscle cell line obtained from embryonic rat thoracic aorta. This nuclear extract comprises a comprehensive array of nuclear proteins, including transcription factors, chromatin remodeling proteins, and various regulatory elements that play crucial roles in gene expression and nuclear organization. The A-10 nuclear extract is particularly valuable for studying cellular mechanisms related to smooth muscle biology, such as vascular development and the regulation of smooth muscle-specific genes. Researchers use this extract to investigate the nuclear signaling pathways and transcriptional networks that smooth muscle cells employ in response to physiological and pathological stimuli. The A-10 nuclear extract enables detailed analysis of protein-DNA interactions, coactivator complexes, and the impact of various signaling cascades on nuclear function. By utilizing this nuclear extract in chromatin immunoprecipitation, electrophoretic mobility shift assays, and other biochemical and molecular biology techniques, scientists can uncover the complex layers of gene regulation and cellular adaptation specific to vascular smooth muscle cells. This research is fundamental in advancing our understanding of vascular biology, providing insights that are crucial for deciphering the molecular basis of vascular diseases and developmental processes in vascular tissues.