8-(4-Chlorophenylthio)-2'-O-methyladenosine 3',5'-cyclic Monophosphate sodium salt is a specific, potent and membrane permeant activator of the Epac cAMP receptor. The compound activates Epac1 and Epac2, the guanine nucleotide-exchange factors (GEFs) for the small GTPases Rap1 and Rap2. 8-CPT-2'-O-Me-cAMP . Na Does not activate protein kinase A (PKA), and is therefore a valuable tool to specifically discriminate between PKA- and Epac-mediated effects. Since a free 2'-ribose hydroxyl group in cyclic AMP is essential for stimulation of PKA, the methylated structure of 8-CPT-2'-O-Me-cAMP . Na is an extremely poor PKA activator. 8-CPT-2'-O-Me-cAMP . Na has a high lipophilicity which allows for good membrane permeability in most biosystems and its increased resistance towards phosphodiesterases prevents rapid hydrolysis.
1. Enserink, J.M., et al. 2002. Nat. Cell Biol. 4: 901-906. PMID: 12402047 2. Kang, G., et al. 2003. J. Biol. Chem. 278: 8279-8285. PMID: 12496249 3. Rangarajan, S., et al. 2003. J. Cell Biol. 160: 487-493. PMID: 12578910
Soluble in water (100 mM), aqueous buffers, DMSO, DMF, and methanol.
Store at -20° C
Epac1: EC5050 = 2.2 µM
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Adobe Acrobat Reader is required to reliably view, print and comment on PDF documents
What is the solubility of sc-202028 in DMSO?
Asked by: SCchem16
Thank you for your question. The compound, sc-202028, is soluble in water, aqueous buffers, DMSO, dimethyl formamide, and methanol. Unfortunately, we do not have the working concentrations. If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Rated 5 out of
BrynBryn, T. et al. (PubMed 16751380) used the Epac cAMP receptor activator, 8-(4-Chlorophenylthio)-2'-O-methyladenosine 3',5'-cyclic Monophosphate sodium salt, to demonstrate that the cyclic AMP-Epac1-Rap1 pathway is dissociated from regulation of effector functions in monocytes but acquires immunoregulatory function in mature macrophages. -SCBT Publication Review
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn