7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin (CPM) is a thiol-reactive fluorescent probe. CPM selectively forms Michael adducts in the presence of hyperreactive sulfhydryls. CPM has been used to monitor the release of thiols, quantitate thiol in microplate reactions, and to distinguish proliferating cancer cells by nucleolar protein staining.
1. Sippel, T.O. 1981. J. Histochem. Cytochem. 29: 1377-1381. PMID: 7320496 2. Liu, G., et al. 1994. J. Biol. Chem. 269: 33028-33034. PMID: 7806531 3. Liu, G., et al. 1994. Mol. Pharmacol. 45: 189-200. PMID: 8114670 4. Feng, W., et al. 1999. Mol. Pharmacol. 55: 821-831. PMID: 10220560 5. Han, M.S., et al. 2004. J. Org. Chem. 69: 2853-2855. PMID: 15074938 6. Ptak, C.P., et al. 2005. Biochemistry. 44: 62-71. PMID: 15628846 7. Khersonsky, O., et al. 2006. Chembiochem. 7: 49-53. PMID: 16329153
Soluble in water (partly), methanol, DMSO, DMF, and chloroform.
Desiccate at -20° C
282.76° C (Predicted)
~618.7° C at 760 mmHg (Predicted)
~1.3 g/cm3 (Predicted)
n20D 1.65 (Predicted)
λex 391 nm, λem 472 nm in methanol
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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Various publications cite the use of 7Various publications cite the use of 7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin (CPM) as a thiol-reactive fluorescent probe. CPM has been used to monitor the release of thiols, quantitate thiol in microplate reactions, and to distinguish proliferating cancer cells by nucleolar protein staining. -SCBT Publication Review
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