7-Chloro-6-fluoro-1-(4-fluorophenyl)-1,4-dihydro-4-oxo-3-quinolinecarboxylic Acid (CAS 98105-79-4)

7-Chloro-6-fluoro-1-(4-fluorophenyl)-1,4-dihydro-4-oxo-3-quinolinecarboxylic acid, a synthetic quinolone derivative, is primarily recognized for its role in inhibiting bacterial DNA gyrase and topoisomerase IV, enzymes crucial for DNA replication, transcription, and repair. The mechanism of action involves the binding of the compound to the DNA-enzyme complex, thereby preventing the supercoiling and uncoiling of DNA necessary for replication and other cellular processes. This inhibition leads to the disruption of bacterial DNA synthesis and ultimately results in cell death. In research, this compound is extensively utilized to study the mechanisms of quinolone action and resistance in bacterial populations. Researchers employ it to investigate the biochemical pathways affected by quinolone inhibition and to explore the structural interactions between the drug and its target enzymes. Studies using this compound have provided significant insights into the genetic mutations that confer resistance to quinolones, helping to explain the molecular basis of resistance mechanisms in bacteria. Moreover, 7-Chloro-6-fluoro-1-(4-fluorophenyl)-1,4-dihydro-4-oxo-3-quinolinecarboxylic acid is used in structural biology to determine the crystal structures of enzyme-drug complexes, enhancing the understanding of drug binding and activity. This compound′s role in research extends to the development of new quinolone derivatives with improved efficacy and reduced resistance, contributing to the broader field of antimicrobial research.