7-Aminoactinomycin D (CAS 7240-37-1)
See product citations (9)
QUICK LINKS
7-Aminoactinomycin D, an analog of the antibiotic actinomycin D (sc-200906), is a fluorescent DNA dye which is useful for quantifying apoptosis. 7-AAD has also been used in studies on cellular necrosis, chromosome analysis and in cell cycle studies. As a fluorescent DNA dye, 7-Aminoactinomycin D is able to intercalate DNA via binding to the GC regions. When used in flow cytometery, 7-Aminoactinomycin D can be used to discriminate between live cells as well as early and late apoptosis. Additionally, 7-Aminoactinomycin D can also be combined with other staining procedures for analysis of intracellular and cell surface antigens enabling multiparametric analysis. When utilizing 7-Aminoactinomycin D with flow cytometery, early and late apoptosis can be studied by varying the concentration of 7-Aminoactinomycin D. During apoptosis, the cellular plasma membrane is altered progressively, which allows the cell membrane to become permeable to 7-Aminoactinomycin D. 7-Aminoactinomycin D staining can also be adapted to include staining with other dyes for intracellular and cell surface antigens. 7-Aminoactinomycin D is an inhibitor of DNA Polymerase and RNA Polymerase.
7-Aminoactinomycin D (CAS 7240-37-1) References
- 7-substituted actinomycin D analogs. Chemical and growth-inhibitory studies. | Sengupta, SK., et al. 1975. J Med Chem. 18: 1175-80. PMID: 1059773
- A novel flow cytometric assay for quantitation and multiparametric characterization of cell-mediated cytotoxicity. | Lecoeur, H., et al. 2001. J Immunol Methods. 253: 177-87. PMID: 11384679
- Phagocytosis of apoptotic cells assessed by flow cytometry using 7-Aminoactinomycin D. | Gaforio, JJ., et al. 2002. Cytometry. 49: 8-11. PMID: 12210605
- Improved staining method for the simultaneous flow cytofluorometric analysis of DNA content, S-phase fraction, and surface phenotype using single laser instrumentation. | Toba, K., et al. 1992. Cytometry. 13: 60-7. PMID: 1372203
- Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry. | Schmid, I., et al. 1992. Cytometry. 13: 204-8. PMID: 1547670
- 7-Aminoactinomycin binding to DNA sequences lacking GpC sites: a thermodynamic and kinetic study. | Biver, T., et al. 2009. Biochemistry. 48: 173-9. PMID: 19072038
- 7-Aminoactinomycin D for apoptosis staining in flow cytometry. | Zembruski, NC., et al. 2012. Anal Biochem. 429: 79-81. PMID: 22796502
- WDR45 contributes to neurodegeneration through regulation of ER homeostasis and neuronal death. | Wan, H., et al. 2020. Autophagy. 16: 531-547. PMID: 31204559
- MIR145-3p promotes autophagy and enhances bortezomib sensitivity in multiple myeloma by targeting HDAC4. | Wu, H., et al. 2020. Autophagy. 16: 683-697. PMID: 31242129
- Necrostatin-1 Prevents Ferroptosis in a RIPK1- and IDO-Independent Manner in Hepatocellular Carcinoma. | Yuk, H., et al. 2021. Antioxidants (Basel). 10: PMID: 34572979
- Mesenchymal Stem Cell Identification After Delayed Cord Clamping. | Smith, ER., et al. 2022. Reprod Sci.. PMID: 36443591
- 7-Amino-actinomycin D as a specific fluorophore for DNA content analysis by laser flow cytometry. | Zelenin, AV., et al. 1984. Cytometry. 5: 348-54. PMID: 6468175
- A rapid method for measuring apoptosis and dual-color immunofluorescence by single laser flow cytometry. | Schmid, I., et al. 1994. J Immunol Methods. 170: 145-57. PMID: 8157993
- Flow cytometric immunofluorescence assay for quantification of cyclobutyldithymine dimers in separate phases of the cell cycle. | Berg, RJ., et al. 1993. Carcinogenesis. 14: 103-6. PMID: 8425255
- Accessibility of 7-aminoactinomycin D to lymphocyte nuclei after paraformaldehyde fixation. | Lindén, E., et al. 1997. Cytometry. 27: 92-5. PMID: 9000590
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
7-Aminoactinomycin D, 1 mg | sc-221210 | 1 mg | $180.00 | |||
7-Aminoactinomycin D, 5 mg | sc-221210A | 5 mg | $408.00 |