6CKine Double Nickase Plasmid (h): sc-403041-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- 6CKine Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- 6CKine Double Nickase Plasmid (h) and 6CKine Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CCL21. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
6CKine Double Nickase Plasmid (h) | sc-403041-NIC | 20 µg | $410.00 | |||
6CKine Double Nickase Plasmid (h2) | sc-403041-NIC-2 | 20 µg | $410.00 |
CCL21 encodes the chemokine 6CKine, a homeostatic CC chemokine that binds CCR7 to direct chemotaxis of naïve T cells, central memory T cells, and mature dendritic cells toward lymphatic vessels and secondary lymphoid organs. Through CCR7-dependent signaling, CCL21 contributes to immune cell trafficking, lymphoid tissue organization, and coordination of antigen presentation and adaptive immune priming. Dysregulated CCL21–CCR7 axis activity has been linked to altered leukocyte infiltration, chronic inflammatory microenvironments, and mechanisms relevant to tumor immune contexture and metastatic dissemination patterns. As a lymphoid chemokine, CCL21 is frequently studied for its roles in lymphangiogenesis-associated immune routing and stromal-immune interactions within tissues.
6CKine Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CCL21 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CCL21. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CCL21 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CCL21-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.