6-Hexadecanoylamino-4-methylumbelliferyl Phosphorylcholine (CAS 904315-61-3)
QUICK LINKS
6-Hexadecanoylamino-4-methylumbelliferyl Phosphorylcholine (HUPC) is a synthetic substrate used in biochemical assays to measure the activity of enzymes such as phospholipases and choline kinases. When 6-Hexadecanoylamino-4-methylumbelliferyl Phosphorylcholine is cleaved by an enzyme that hydrolyzes phospholipids or phosphorylcholine-containing compounds, the MU chromophore is released and emits a bright blue fluorescence that can be detected using a fluorescence spectrophotometer. The rate of MU fluorescence increase is proportional to the enzyme activity, and therefore, 6-Hexadecanoylamino-4-methylumbelliferyl Phosphorylcholine can be used to quantify the enzyme activity in biological samples. 6-Hexadecanoylamino-4-methylumbelliferyl Phosphorylcholine is particularly useful in the detection and measurement of phospholipase A2 (PLA2) activity, an enzyme that catalyzes the hydrolysis of phospholipids to produce arachidonic acid and lysophospholipids.
6-Hexadecanoylamino-4-methylumbelliferyl Phosphorylcholine (CAS 904315-61-3) References
- Ceramide in rafts (detergent-insoluble fraction) mediates cell death in neurotumor cell lines. | Kilkus, J., et al. 2003. J Neurosci Res. 72: 65-75. PMID: 12645080
- Regulation of sphingomyelinases in cells of the oligodendrocyte lineage. | Testai, FD., et al. 2004. J Neurosci Res. 75: 66-74. PMID: 14689449
- Acid sphingomyelinase and inhibition by phosphate ion: role of inhibition by phosphatidyl-myo-inositol 3,4,5-triphosphate in oligodendrocyte cell signaling. | Testai, FD., et al. 2004. J Neurochem. 89: 636-44. PMID: 15086520
- Potent inhibition of acid sphingomyelinase by phosphoinositide analogues. | Roth, AG., et al. 2009. Chembiochem. 10: 2367-74. PMID: 19688786
- Development of carbohydrate-derived inhibitors of acid sphingomyelinase. | Roth, AG., et al. 2010. Bioorg Med Chem. 18: 939-44. PMID: 20005726
- A high-throughput sphingomyelinase assay using natural substrate. | Xu, M., et al. 2012. Anal Bioanal Chem. 404: 407-14. PMID: 22710568
- Differential activation of acid sphingomyelinase and ceramide release determines invasiveness of Neisseria meningitidis into brain endothelial cells. | Simonis, A., et al. 2014. PLoS Pathog. 10: e1004160. PMID: 24945304
- Neutral sphingomyelinase 2 is a key factor for PorB-dependent invasion of Neisseria gonorrhoeae. | Faulstich, M., et al. 2015. Cell Microbiol. 17: 241-53. PMID: 25224994
- Neutral sphingomyelinase in physiological and measles virus induced T cell suppression. | Mueller, N., et al. 2014. PLoS Pathog. 10: e1004574. PMID: 25521388
- Prenatal Diagnosis of Lysosomal Storage Disorders Using Chorionic Villi. | Verma, J., et al. 2017. Methods Mol Biol. 1594: 265-291. PMID: 28456990
- The Activity of the Neutral Sphingomyelinase Is Important in T Cell Recruitment and Directional Migration. | Collenburg, L., et al. 2017. Front Immunol. 8: 1007. PMID: 28871263
- Neutral Sphingomyelinase-2 (NSM 2) Controls T Cell Metabolic Homeostasis and Reprogramming During Activation. | De Lira, MN., et al. 2020. Front Mol Biosci. 7: 217. PMID: 33088808
- Lysosomal lipid alterations caused by glucocerebrosidase deficiency promote lysosomal dysfunction, chaperone-mediated-autophagy deficiency, and alpha-synuclein pathology. | Navarro-Romero, A., et al. 2022. NPJ Parkinsons Dis. 8: 126. PMID: 36202848
- Discovery of Novel N-Hydroxy-1,2,4-oxadiazole-5-formamides as ASM Direct Inhibitors for the Treatment of Atherosclerosis. | Yang, K., et al. 2023. J Med Chem. 66: 2681-2698. PMID: 36786607
- Unexpected phenotypic and molecular changes of combined glucocerebrosidase and acid sphingomyelinase deficiency. | Keatinge, M., et al. 2023. Dis Model Mech.. PMID: 36951087
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
6-Hexadecanoylamino-4-methylumbelliferyl Phosphorylcholine, 1 mg | sc-490447 | 1 mg | $480.00 | |||
6-Hexadecanoylamino-4-methylumbelliferyl Phosphorylcholine, 2 mg | sc-490447A | 2 mg | $815.00 | |||
6-Hexadecanoylamino-4-methylumbelliferyl Phosphorylcholine, 5 mg | sc-490447B | 5 mg | $1530.00 | |||
6-Hexadecanoylamino-4-methylumbelliferyl Phosphorylcholine, 10 mg | sc-490447C | 10 mg | $2950.00 |