5β-Cholanic acid-3α,12α-diol 3-acetate methyl ester (CAS 27240-83-1)

5β-Cholanic acid-3α,12α-diol 3-acetate methyl ester is a bile acid derivative extensively utilized in biochemical research to investigate the regulation and function of bile acids within cellular processes. This compound has been pivotal in studying the interaction with nuclear receptors, particularly the farnesoid X receptor (FXR) and liver X receptor (LXR), which are key regulators of bile acid metabolism, lipid homeostasis, and cholesterol transport. Research utilizing this ester has provided insights into the modulation of gene expression involved in bile acid synthesis, transport, and detoxification pathways. Additionally, it has been used to explore the feedback mechanisms that control bile acid synthesis, highlighting its role in maintaining bile acid homeostasis. Studies have also leveraged this compound to examine its effects on hepatocyte function, bile acid-induced cellular stress, and apoptosis. Its impact on the expression of transport proteins and enzymes critical for bile acid conjugation and elimination has been a focal point in understanding liver physiology and pathology. Furthermore, recent research has delved into its influence on metabolic pathways, offering a deeper comprehension of the crosstalk between bile acids and metabolic regulators. This chemical continues to be a valuable tool for elucidating complex biochemical pathways and regulatory networks in cellular and molecular biology.