5(6)-Carboxy-2prime,7prime-dichlorofluorescein diacetate CAS: 127770-45-0
MF: C25H14Cl2O9
MW: 529.28
A fluorescent substrate and probe.

5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate (CAS 127770-45-0)

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Alternate Names: 5(6)-CDCFDA
Application: 5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate is a fluorescent substrate and probe
CAS Number: 127770-45-0
Purity: ≥97%
Molecular Weight: 529.28
Molecular Formula: C25H14Cl2O9
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
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5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate is an amine–reactive fluorescein diacetate (FDA) derivative used to prepare a variety of FDA conjugates. It is a non-fluorescent molecule that diffuses into cells and is hydrolyzed by intracellular non-specific esterases, yielding a fluorescent probe. The probe accumulates exclusively in cells with intact cell membranes. 5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate does not stain dead cells but it can be used for monitoring cells by flow cytometry or fluorescence microscopy. The probe is also frequently used to monitor reactive oxygen species (ROS) generation in live cells.

Physical State :
Solid
Storage :
Store at -20° C
Melting Point :
210° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
PubChem CID :
16211578
SMILES :
CC(=O)OC1=C(C=C2C(=C1)OC3=CC(=C(C=C3C24C5=C(C=C(C=C5)C(=O)O)C(=O)O4)Cl)OC(=O)C)Cl.CC(=O)OC1=C(C=C2C(=C1)OC3=CC(=C(C=C3C24C5=C(C=CC(=C5)C(=O)O)C(=O)O4)Cl)OC(=O)C)Cl

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Certificate of Analysis

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