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5-(Iodoacetamido)fluorescein (CAS 63368-54-7)

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Alternate Names:
5-IAF
Application:
5-(Iodoacetamido)fluorescein is a thiol reactive fluorescein derivative
CAS Number:
63368-54-7
Molecular Weight:
515.25
Molecular Formula:
C22H14INO6
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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5-(Iodoacetamido)fluorescein is a molecule used in biochemical research for labeling proteins, peptides, and other biomolecules. Its ability to covalently attach to thiol groups in cysteine residues through its iodoacetamide functional group underlies its frequent application in fluorescence microscopy and flow cytometry. This compound is particularly valuable for investigating protein structure and dynamics, as the attached fluorescein moiety provides a fluorescent signal that can be detected and quantified. In protein-protein interaction studies, 5-(Iodoacetamido)fluorescein facilitates the visualization of complex formations, thereby aiding in the elucidation of molecular mechanisms. It is also used in the study of enzymatic activities and conformational changes in proteins, as the fluorescent properties of the compound change upon binding, providing a measure of the biochemical processes in real-time.


5-(Iodoacetamido)fluorescein (CAS 63368-54-7) References

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  3. Light-induced proton slip and proton leak at the thylakoid membrane.  |  Richter, M., et al. 2004. J Plant Physiol. 161: 1325-37. PMID: 15658803
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  7. Inhibition of tubulin polymerization by hypochlorous acid and chloramines.  |  Landino, LM., et al. 2011. Free Radic Biol Med. 50: 1000-8. PMID: 21256958
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  9. Hypothiocyanous acid oxidation of tubulin cysteines inhibits microtubule polymerization.  |  Clark, HM., et al. 2014. Arch Biochem Biophys. 541: 67-73. PMID: 24215946
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Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

5-(Iodoacetamido)fluorescein, 25 mg

sc-214304
25 mg
$162.00

5-(Iodoacetamido)fluorescein, 100 mg

sc-214304A
100 mg
$537.00

5-(Iodoacetamido)fluorescein, 250 mg

sc-214304B
250 mg
$1200.00