5-Iodo-Indirubin-3′-monoxime

5-Iodo-Indirubin-3′-monoxime is a synthetic derivative of indirubin. This chemical is part of the indirubin family, known for their potent inhibitory activity against various kinases, particularly cyclin-dependent kinases (CDKs) and glycogen synthase kinase-3 (GSK-3). The mechanism of action of 5-Iodo-Indirubin-3′-monoxime involves its interaction with the ATP-binding sites of these kinases. By binding to these sites, it competitively inhibits the kinases′ ability to phosphorylate their respective substrates. This inhibition results in the modulation of various cellular processes controlled by these enzymes, such as cell cycle progression and signal transduction pathways. The addition of the iodine atom at the 5-position on the indirubin molecule enhances its specificity and potency as a kinase inhibitor. In research, 5-Iodo-Indirubin-3′-monoxime has been extensively used to study the regulation of cell cycle and cell division, particularly in cancer cell lines. It has been a valuable tool for dissecting the molecular mechanisms underlying cellular responses to kinase inhibition, especially in understanding how these responses can be manipulated to control cell growth and proliferation.