5-Hydroxyindole-3-acetic acid-D5 (CAS 81587-11-3)

5-Hydroxyindole-3-acetic acid-D5 (5-HIAA-D5) is a deuterium-labeled derivative of 5-hydroxyindole-3-acetic acid, primarily used as an internal standard in analytical chemistry for quantifying levels of its unlabeled counterpart via mass spectrometry techniques. This isotopically labeled version of 5-HIAA provides an essential tool for ensuring accuracy and precision in the measurement processes, particularly in complex biological matrices where exact quantification is crucial. The presence of deuterium atoms minimizes the exchange interactions that often occur in the hydrogen positions, thereby stabilizing the molecule against isotopic exchange and degradation. This attribute is particularly beneficial in studies that require rigorous, reproducible analytical data, and it supports robust method validation by matching the physical and chemical properties of the analyte, except for the slight mass difference due to the presence of deuterium. Thus, 5-Hydroxyindole-3-acetic acid-D5 plays a critical role in facilitating research and development in fields that rely on precise quantitative analysis of chemical compounds.