5-Ethynyl-2′-deoxyuridine (CAS 61135-33-9)
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5-Ethynyl-2′-deoxyuridine is also known as EdU, 5-EdU, and 2′-Deoxy-5-ethynyluridine. 5-Ethynyl-2′-deoxyuridine is an extensively used nucleoside analog of thymidine used as a probe for DNA synthesis. 5-Ethynyl-2′-deoxyuridine is known as an toxic anti-metabolite, which potentially causes DNA instability, necrosis and cell-cycle arrest therefore 5-Ethynyl-2′-deoxyuridine is often used for short-term DNA synthesis in tissue culture and living organisms where prolonged cell survival is not required. 5-Ethynyl-2′-deoxyuridine can also be used to monitor de novo DNA synthesis through click chemistry. Like 5-bromo-2′-deoxyuridine (sc-290815), 5-Ethynyl-2′-deoxyuridine is incorporated into DNA during active DNA synthesis and can then be detected using a fluorescent azide probe and copper catalysis using 1,3-dipolar cycloaddition.
5-Ethynyl-2′-deoxyuridine (CAS 61135-33-9) References
- 5-Alkynyl-2'-deoxyuridines: chromatography-free synthesis and cytotoxicity evaluation against human breast cancer cells. | Meneni, S., et al. 2007. Bioorg Med Chem. 15: 3082-8. PMID: 17336074
- Detection of S-phase cell cycle progression using 5-ethynyl-2'-deoxyuridine incorporation with click chemistry, an alternative to using 5-bromo-2'-deoxyuridine antibodies. | Buck, SB., et al. 2008. Biotechniques. 44: 927-9. PMID: 18533904
- Cell type specific applicability of 5-ethynyl-2'-deoxyuridine (EdU) for dynamic proliferation assessment in flow cytometry. | Diermeier-Daucher, S., et al. 2009. Cytometry A. 75: 535-46. PMID: 19235202
- Synthesis and enzymatic incorporation of modified deoxyuridine triphosphates. | Borsenberger, V., et al. 2009. Org Biomol Chem. 7: 3826-35. PMID: 19707689
- Evaluation of 5-ethynyl-2'-deoxyuridine staining as a sensitive and reliable method for studying cell proliferation in the adult nervous system. | Zeng, C., et al. 2010. Brain Res. 1319: 21-32. PMID: 20064490
- Thymidine analogues for tracking DNA synthesis. | Cavanagh, BL., et al. 2011. Molecules. 16: 7980-93. PMID: 21921870
- Dynamic metabolic labeling of DNA in vivo with arabinosyl nucleosides. | Neef, AB. and Luedtke, NW. 2011. Proc Natl Acad Sci U S A. 108: 20404-9. PMID: 22143759
- The sub-cellular localization of Sulfolobus DNA replication. | Gristwood, T., et al. 2012. Nucleic Acids Res. 40: 5487-96. PMID: 22402489
- Proliferation assays (BrdU and EdU) on skeletal tissue sections. | Mead, TJ. and Lefebvre, V. 2014. Methods Mol Biol. 1130: 233-243. PMID: 24482177
- 5-Ethynyl-2'-deoxycytidine and 5-ethynyl-2'-deoxyuridine are differentially incorporated in cells infected with HSV-1, HCMV, and KSHV viruses. | Manska, S., et al. 2020. J Biol Chem. 295: 5871-5890. PMID: 32205447
- 5-Ethynyl-2'-Deoxyuridine/Phospho-Histone H3 Dual-Labeling Protocol for Cell Cycle Progression Analysis in Drosophila Neural Stem Cells. | Chippalkatti, R. and Suter, B. 2021. J Vis Exp.. PMID: 34028440
- EDU (5-Ethynyl-2'-Deoxyuridine)-Coupled Fluorescence-Intensity Analysis: Determining Absolute Parameters of the Cell Cycle. | Ferreira, JA., et al. 2021. Methods Mol Biol. 2329: 165-177. PMID: 34085222
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
5-Ethynyl-2′-deoxyuridine, 250 mg | sc-284628 | 250 mg | $220.00 | |||
5-Ethynyl-2′-deoxyuridine, 1 g | sc-284628A | 1 g | $582.00 | |||
5-Ethynyl-2′-deoxyuridine, 10 g | sc-284628B | 10 g | $3641.00 |