(−)-5-Bromouridine (CAS 957-75-5)

(−)-5-Bromouridine, a halogenated nucleoside analog, has garnered significant interest in scientific research due to its unique properties and versatile applications. One of its primary research applications lies in molecular biology, where it serves as a valuable tool for studying RNA dynamics, structure, and function. (−)-5-Bromouridine can be incorporated into nascent RNA during transcription, allowing researchers to label newly synthesized RNA molecules in live cells. This feature has been exploited in techniques such as bromouridine immunoprecipitation followed by sequencing (BrU-IP-seq) and bromouridine labeling and capture (BrdU-seq), enabling the high-resolution mapping of RNA synthesis rates, RNA processing events, and RNA-protein interactions on a genome-wide scale. Moreover, (−)-5-Bromouridine labeling has been employed in pulse-chase experiments to track the turnover rates of specific RNA transcripts and investigate the dynamics of RNA metabolism under various physiological conditions or perturbations. Additionally, (−)-5-Bromouridine incorporation has been utilized in the synthesis of modified RNA molecules for applications in RNA nanotechnology and aptamer development. Ongoing research continues to refine labeling protocols, optimize detection methods, and explore new avenues for harnessing (−)-5-Bromouridine as a versatile tool in RNA biology, with implications for deciphering the complexities of gene expression regulation and advancing RNA-based technologies.