5-Bromo-PAPS (CAS 679787-08-7)

5-Bromo-PAPS is a chemical compound that is utilized in various research applications within the fields of biochemistry and molecular biology. This compound is a derivative of 3′-Phosphoadenosine-5′-phosphosulfate (PAPS), which is a universal sulfate donor in enzymatic sulfation reactions. The bromine atom introduced into the molecule provides a handle for radioisotope labeling or can serve as a site for further chemical modifications. Researchers use 5-Bromo-PAPS to study sulfotransferase enzymes, as it acts as a substrate analog to investigate the mechanisms of sulfation processes, substrate specificity, and enzyme kinetics. It is also utilized in the development of assays for the identification and characterization of inhibitors of sulfotransferases, which are important for understanding the regulation of sulfation in physiological and pathological conditions. The modification with bromine allows for advanced techniques such as X-ray crystallography to be employed to elucidate the structural details of enzyme-substrate interactions.