Molecular structure of 5-Bromo-6-chloro-3-indolyl phosphate, p-toluidine salt, CAS Number: 6769-80-8
5-Bromo-6-chloro-3-indolyl phosphate, p-toluidine salt (CAS 6769-80-8)
Alternate Names:
(5-bromo-6-chloro-1H-indol-3-yl) dihydrogen phosphate; 4-methylaniline
Application:
5-Bromo-6-chloro-3-indolyl phosphate, p-toluidine salt is a staining substance for alkaline phosphatase activity
CAS Number:
6769-80-8
Purity:
≥98%
Molecular Weight:
433.63
Molecular Formula:
C8H6BrClNO4P•C7H9N
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
5-Bromo-6-chloro-3-indoxyl phosphate p-toluidine salt, also known as Magenta-phos p-toluidine salt, is a histochemical substance used for visualizing areas of alkaline phosphatase activity. Hydrolysis of the phosphate group on 5-Bromo-6-chloro-3-indoxyl phosphate by alkaline phosphatase generates an insoluble indigo compound. The indoxyl species resulting from phosphate hydrolysis of related compounds can be reacted with tetrazolium salts, reducing the tetrazolium to a colorful insoluble formazan species. A solubilization technique involving reduction of the insoluble hydrolytic product of the closely related 5-Bromo-4-chloro-3-indoxyl phosphate permitted the use of this substrate in quantitative ELISA experiments. 5-Bromo-6-chloro-3-indolyl phosphate, p-toluidine salt is a substrate of TNAP.
5-Bromo-6-chloro-3-indolyl phosphate, p-toluidine salt (CAS 6769-80-8) References
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Substrate of:
HRP and AP, and TNAP.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
5-Bromo-6-chloro-3-indolyl phosphate, p-toluidine salt, 100 mg | sc-221017 | 100 mg | $188.00 | |||
5-Bromo-6-chloro-3-indolyl phosphate, p-toluidine salt, 250 mg | sc-221017A | 250 mg | $350.00 |