5-Bromo-4-chloro-3-indolyl α-D-N-acetylneuraminic acid sodium salt (CAS 160369-85-7)
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5-Bromo-4-chloro-3-indolyl alpha-D-N-acetylneuraminic acid sodium salt is a chromogenic substrate for neuraminidases. These are enzymes that cleave glycosidic linkages of neuraminic acids. Upon hydrolysis by neuraminidase, halogenated indol-3-ol is released and undergoes rapid aerobic oxidation to the dark blue pigment 5,5′-dibromo-4-4′-dichloroindigo, which can be used to quantify neuraminidase activity. This product can be used to facilitate the screening of bacterial colonies or plaques for the detection of either natural or mutant neuraminidase activity. Preliminary kinetic studies indicate that this compound is a good substrate for neuraminidase and is quite stable under identical conditions in the absence of enzyme. These results suggest that 5-Bromo-4-chloro-3-indolyl alpha-D-N-acetylneuraminic acid sodium salt can be useful to screen for bacterially-encoded enzyme production directly on agar plates.
5-Bromo-4-chloro-3-indolyl α-D-N-acetylneuraminic acid sodium salt (CAS 160369-85-7) References
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Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
5-Bromo-4-chloro-3-indolyl α-D-N-acetylneuraminic acid sodium salt, 10 mg | sc-217158 | 10 mg | $256.00 | |||
5-Bromo-4-chloro-3-indolyl α-D-N-acetylneuraminic acid sodium salt, 50 mg | sc-217158A | 50 mg | $549.00 |