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5-Bromo-4-chloro-3-indolyl α-D-mannopyranoside (CAS 125229-64-3)

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Alternate Names:
X-man
Application:
5-Bromo-4-chloro-3-indolyl α-D-mannopyranoside is a halogenated derivative of α-D-mannopyranoside
CAS Number:
125229-64-3
Molecular Weight:
408.63
Molecular Formula:
C14H15BrClNO6
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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5-Bromo-4-chloro-3-indolyl α-D-mannopyranoside is a substrate to detect the activity of α-mannosidase enzymes. When added to a reaction mixture, 5-Bromo-4-chloro-3-indolyl α-D-mannopyranoside is hydrolyzed by α-mannosidase, resulting in the release of a blue dye. This allows for the visualization and quantification of α-mannosidase activity in various biological samples. The mechanism of action involves the cleavage of the glycosidic bond between the indolyl moiety and the mannose residue by the α-mannosidase enzyme. This results in the formation of a blue-colored product, which can be easily detected and measured using spectrophotometric or fluorometric methods. 5-Bromo-4-chloro-3-indolyl α-D-mannopyranoside ′s function as a substrate for α-mannosidase may be useful in studying the role of this enzyme in various biological processes.


5-Bromo-4-chloro-3-indolyl α-D-mannopyranoside (CAS 125229-64-3) References

  1. A long-wavelength fluorescent substrate for continuous fluorometric determination of alpha-mannosidase activity: resorufin alpha-D-mannopyranoside.  |  Coleman, DJ., et al. 2010. Anal Biochem. 399: 7-12. PMID: 20026005
  2. A multi-substrate approach for functional metagenomics-based screening for (hemi)cellulases in two wheat straw-degrading microbial consortia unveils novel thermoalkaliphilic enzymes.  |  Maruthamuthu, M., et al. 2016. BMC Genomics. 17: 86. PMID: 26822785
  3. Halotolerant microbial consortia able to degrade highly recalcitrant plant biomass substrate.  |  Cortes-Tolalpa, L., et al. 2018. Appl Microbiol Biotechnol. 102: 2913-2927. PMID: 29397428

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

5-Bromo-4-chloro-3-indolyl α-D-mannopyranoside, 25 mg

sc-217157
25 mg
$216.00