5-Bromo-2'-deoxyuridine is a thymidine analog. Useful for the study of DNA synthesis where it is incorporated into DNA in place of thymidine. 5-Bromo-2'-deoxyuridine can be used to label DNA. The resulting DNA can be used in conjunction with BrdU Monoclonal Antibody for the immunohistochemical analysis of cell proliferation.
Garabedian, E.M., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 15382. Cattoretti, G., et al. 1993. J. Pathol. 171, 83. Fukuda, K., et al. 1990. Anal. Quant. Cytol. Histol. 12, 135.
Soluble in water (10 mg/mL), NH4OH (0.1 M) at 20° C, DMF, DMSO, and sodium hydroxide.
Store at -20° C
191-194° C (lit.)(dec.)
n20D 1.65 (Predicted)
α25/D +22.9°, c = 1% in water; α20/D +31°, c = 1 in 0.1N HCl
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What is the recommended dose of inoculation?
Asked by: DavRoq
Thank you for your question. The researchers of the following publication cite 0.5 mg/ml BDU.
Answered by: Technical Support
Date published: 2017-07-20
Podria indicarme con que referencias anticuerpos es compatible este producto: 5-Bromo-2'-deoxyuridinesc-290815
Asked by: Cassio
Thank you very much for your question. 5-Bromo-2'-deoxyuridine can be used to label DNA. The resulting DNA can be used in conjunction with BrdU Monoclonal Antibody for the immunohistochemical analysis of cell proliferation. We currently offer different antibodies for the detection of BrdU, e.g. our most popular antibody is BrdU Antibody (IIB5) sc-32323. However, for the detection of BrdU incorporated into DNA during S-phase of a cell cycle by IF, IHC(P) and FCM we recommend the BrdU Antibody (MoBU-1): sc-51514.
Answered by: Technical Support Europe
Date published: 2017-07-14
What is the appearance of the compound?
Asked by: two2igm05
Thank you for your question. This chemical, 5-Bromo-2'-deoxyuridine: sc-290815, has the appearance of a white solid.
Rated 5 out of
Works great!We have been using this BrdU for more than a year. It has been working perfectly without any problem.
Date published: 2019-04-29
Rated 5 out of
Numerous publications cite the use of 5Numerous publications cite the use of 5-Bromo-2'-deoxyuridine, a thymidine analog, to label DNA, typically in cell proliferation studies. -SCBT Publication Review
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