Date published: 2025-10-21

1-800-457-3801

SCBT Portrait Logo
Seach Input

5(6)-Carboxyfluorescein (CAS 72088-94-9)

0.0(0)
Write a reviewAsk a question

Application:
5(6)-Carboxyfluorescein is used in the preparation of fluorescent peptide and protein conjugates
CAS Number:
72088-94-9
Molecular Weight:
376.32
Molecular Formula:
C21H12O7
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

QUICK LINKS

5(6)-Carboxyfluorescein, also referred to as 5-FAM, is a highly utilized fluorescent dye derived from fluorescein, a fluorescent compound. In scientific applications, 5(6)-Carboxyfluorescein finds widespread applications, particularly as a fluorescent marker for proteins, nucleic acids, and other molecules. It proves DNA and RNA detection, as well as in flow cytometry and cell sorting techniques. As a fluorescent dye, 5(6)-Carboxyfluorescein is excited by light, emitting a fluorescent signal upon exposure. This signal can be captured and analyzed using microscopes or other imaging devices.


5(6)-Carboxyfluorescein (CAS 72088-94-9) References

  1. Vital staining with 5(6)-carboxyfluorescein in the feeding system of Lymnaea and the selective photoinactivation of the filled neurones.  |  Kemenes, G., et al. 1992. Acta Biol Hung. 43: 351-60. PMID: 1284362
  2. Preparation of 5- and 6-(aminomethyl)fluorescein.  |  Mattingly, PG. 1992. Bioconjug Chem. 3: 430-1. PMID: 1420443
  3. Photoinactivation of neurones axonally filled with the fluorescent dye 5(6)-carboxyfluorescein in the pond snail, Lymnaea stagnalis.  |  Kemenes, G., et al. 1991. J Neurosci Methods. 39: 207-16. PMID: 1787741
  4. Maturation of the corneal endothelial tight junction.  |  Stiemke, MM., et al. 1991. Invest Ophthalmol Vis Sci. 32: 2757-65. PMID: 1894473
  5. Polyelectrolyte coated PLGA nanoparticles: templation and release behavior.  |  Zhou, J., et al. 2009. Macromol Biosci. 9: 326-35. PMID: 19089871
  6. Effect of 2.45 GHz microwave radiation on permeability of unilamellar liposomes to 5(6)-carboxyfluorescein. Evidence of non-thermal leakage.  |  Saalman, E., et al. 1991. Biochim Biophys Acta. 1064: 124-30. PMID: 2025632
  7. PAMAM dendrimer-induced aggregation of 5(6)-carboxyfluorescein.  |  Bonizzoni, M., et al. 2012. J Org Chem. 77: 1258-66. PMID: 22145833
  8. A versatile multicomponent assembly via β-cyclodextrin host-guest chemistry on graphene for biomedical applications.  |  Dong, H., et al. 2013. Small. 9: 446-56. PMID: 23047287
  9. Controlled release of hydrophilic guest molecules from photoresponsive nucleolipid vesicles.  |  Sun, Y., et al. 2013. ACS Appl Mater Interfaces. 5: 6232-6. PMID: 23758265
  10. Phosphorylation regulates proteolytic efficiency of TEV protease detected by a 5(6)-carboxyfluorescein-pyrene based fluorescent sensor.  |  He, YH., et al. 2016. Talanta. 150: 340-5. PMID: 26838417
  11. Embedding calix[4]resorcinarenes in liposomes: Experimental and computational investigation of the effect of resorcinarene inclusion on liposome properties and stability.  |  Zappacosta, R., et al. 2019. Biochim Biophys Acta Biomembr. 1861: 1252-1259. PMID: 31028721
  12. Temperature dependence of membrane ion conductance analyzed by using the amphiphilic anion 5/6-carboxyfluorescein.  |  Bramhall, J., et al. 1987. Biochemistry. 26: 6330-40. PMID: 3427008
  13. Engineering caveolin-mediated endocytosis in Saccharomyces cerevisiae.  |  Zhang, Q., et al. 2022. Synth Syst Biotechnol. 7: 1056-1063. PMID: 35845314
  14. Archaebacterial lipid models: stable liposomes from 1-alky1-2-phytanyl-sn-glycero-3-phosphocholines.  |  Yamauchi, K., et al. 1996. Biochim Biophys Acta. 1283: 163-9. PMID: 8809095

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

5(6)-Carboxyfluorescein, 5 g

sc-221067
5 g
$249.00