5(6)-Carboxy-X-rhodamine N-succinimidyl ester (CAS 114616-32-9)

5(6)-Carboxy-X-rhodamine N-succinimidyl ester (5(6)-ROX-NSE) is a fluorescent dye widely employed in numerous applications, spanning scientific research to laboratory experiments. Belonging to the N-hydroxy succinimide (NHS) ester family of dyes, it finds utility in diverse methods and techniques. This highly fluorescent dye, 5(6)-Carboxy-X-rhodamine N-succinimidyl ester, serves as useful for detecting proteins, nucleic acids, and other biomolecules. It plays a pivotal role in visualizing cellular components, including organelles, and facilitates the quantification of proteins and other molecules. Within scientific research, 5(6)-ROX-NSE holds multifaceted applications. It enables the detection and quantification of proteins, nucleic acids, and other biomolecules. Moreover, the dye contributes to the identification of cellular components, such as organelles, and aids in the quantification of proteins and other molecules. Its significance extends to gene expression analysis, as it allows for the detection and quantification of mRNA and other transcripts. Additionally, the dye proves instrumental in studying protein-protein interactions, offering the ability to detect and measure the binding of proteins to one another. Mechanistically, 5(6)-Carboxy-X-rhodamine N-succinimidyl ester functions by binding to the target molecule and generating a fluorescent signal. Through non-covalent interactions, the dye forms a bond with the target molecule and, when excited by light, emits a fluorescent signal. The intensity of this signal directly correlates with the concentration of the target molecule. Consequently, the dye serves as a reliable means to detect and quantify proteins, nucleic acids, and other biomolecules by binding to them and producing a discernible fluorescent signal.