Molecular structure of 5,5′-Dithio-bis-(2-nitrobenzoic Acid), CAS Number: 69-78-3
5,5′-Dithio-bis-(2-nitrobenzoic Acid) (CAS 69-78-3)
See product citations (3)
Alternate Names:
DTNB; Ellman′s Reagent
Application:
5,5′-Dithio-bis-(2-nitrobenzoic Acid) is a sulfhydryl reagent used to characterize reactive thiol groups
CAS Number:
69-78-3
Purity:
≥99%
Molecular Weight:
396.35
Molecular Formula:
C14H8N2O8S2
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
5,5′-Dithio-bis-(2-nitrobenzoic Acid) is a sulfhydryl reagent used to characterize reactive thiol groups. Used for measuring the free sulfhydryl content in proteins, peptides, and tissues. Also useful in photometric determination of thiols. 5,5′-Dithio-bis-(2-nitrobenzoic Acid) also acts as a blocker of thiol/disulfide exchange on cell membranes.
5,5′-Dithio-bis-(2-nitrobenzoic Acid) (CAS 69-78-3) References
- Reactivation and refolding of a partially folded creatine kinase modified by 5,5'-dithio-bis(2-nitrobenzoic acid). | Yang, Y., et al. 1999. Biochem Biophys Res Commun. 259: 450-4. PMID: 10362528
- Reversible modification of the sulfhydryl groups of Escherichia coli succinic thiokinase with methanethiolating reagents, 5.5'-Dithio-bis(2-nitrobenzoic acid), p-hydroxymercuribenzoate, and ethylmercurithiosalicylate. | Nishimura, JS., et al. 1975. Arch Biochem Biophys. 170: 461-7. PMID: 1103736
- The effect of pralidoxime chloride in the assay of acetylcholinesterase using 5,5'-dithio-bis(2-nitrobenzoic acid) (Ellman's reagent). | Nadarajah, B. 1992. J Anal Toxicol. 16: 192-3. PMID: 1522715
- [Determination of human serum glutathione peroxidase activity, by using hydrogen peroxide and 5,5'-dithio-bis(2-nitrobenzoic acid)]. | Razygraev, AV. and Arutiunian, AV. 2006. Klin Lab Diagn. 13-6. PMID: 16875124
- Assay for quantitative determination of glutathione and glutathione disulfide levels using enzymatic recycling method. | Rahman, I., et al. 2006. Nat Protoc. 1: 3159-65. PMID: 17406579
- 5,5'-Dithio-bis(2-nitrobenzoic acid) modification of cysteine improves the crystal quality of human chloride intracellular channel protein 2. | Mi, W., et al. 2008. Biochem Biophys Res Commun. 368: 919-22. PMID: 18280248
- 2-nitrobenzoate 2-nitroreductase (NbaA) switches its substrate specificity from 2-nitrobenzoic acid to 2,4-dinitrobenzoic acid under oxidizing conditions. | Kim, YH., et al. 2013. J Bacteriol. 195: 180-92. PMID: 23123905
- Selective optical sensing of biothiols with Ellman's reagent: 5,5'-Dithio-bis(2-nitrobenzoic acid)-modified gold nanoparticles. | Güçlü, K., et al. 2013. Anal Chim Acta. 794: 90-8. PMID: 23972980
- Sensitive and selective detection of trivalent chromium using Hyper Rayleigh Scattering with 5,5'-dithio-bis-(2-nitrobenzoic acid)-modified gold nanoparticles. | Hughes, SI., et al. 2013. Sens Actuators B Chem. 178: 514-519. PMID: 24604926
- [Thiol peroxidase activities in rat blood plasma determined with hydrogen peroxide and 5,5`-dithio-bis(2-nitrobenzoic acid)]. | Razygraev, AV., et al. 2016. Biomed Khim. 62: 431-8. PMID: 27562997
- Spectrophotometric determination of reaction rates and kinetic parameters of a BAHD acyltransferase using DTNB (5,5'-dithio-bis-[2-nitrobenzoic acid]). | Sullivan, ML. and Bonawitz, ND. 2018. Plant Sci. 269: 148-152. PMID: 29606213
- Fluorescence biosensor based on silicon quantum dots and 5,5'-dithiobis-(2-nitrobenzoic acid) for thiols in living cells. | Huang, BH., et al. 2020. Spectrochim Acta A Mol Biomol Spectrosc. 229: 117972. PMID: 31891868
- Kinetics of the reaction of baker's yeast glucose-6-phosphate dehydrogenase with 5,5'-dithiobis(2-nitrobenzoic acid). | Adediran, SA. and Gbadegesin, MR. 1995. Arch Biochem Biophys. 322: 39-42. PMID: 7574692
- Selective reactivity of rhodanese sulfhydryl groups with 5,5'-dithio-bis(2-nitrobenzoic acid). | Pensa, B., et al. 1977. Biochim Biophys Acta. 484: 368-74. PMID: 911854
- HPLC determination of biologically active thiols using pre-column derivatisation with 5,5'-dithio-(bis-2-nitrobenzoic acid). | Russell, J., et al. 1997. J Pharm Biomed Anal. 15: 1757-63. PMID: 9260673
Inhibitor of:
CD39, GLYAT, Kell, KRTAP12-2, LAAO, Mcsp, PDI, Selenoprotein M, SPTRX-3, TXNDC1, TXNDC14, and ZIP3.Activator of:
β-Mercaptoethanol, and TrxR3.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
5,5′-Dithio-bis-(2-nitrobenzoic Acid), 5 g | sc-359842 | 5 g | $80.00 |