4-(Trifluoromethyl)umbelliferyl phosphate disodium salt (CAS 352525-17-8)

4-(Trifluoromethyl)umbelliferyl phosphate disodium salt (TFMUP) is experimentally used as a fluorogenic substrate for alkaline phosphatases. 4-(Trifluoromethyl)umbelliferyl phosphate disodium salt holds significant importance as a reagent employed across diverse scientific applications. 4-(Trifluoromethyl)umbelliferyl phosphate disodium salt has played a role in exploring the activity of key enzymes, such as phospholipase A2 (PLA2), phospholipase C (PLC), and protein kinase C (PKC). Moreover, it has facilitated investigations into the impact of various compounds on the enzymatic activity of these key players. 4-(Trifluoromethyl)umbelliferyl phosphate disodium salt has also been instrumental in studying the effects of diverse compounds on lipid and protein metabolism. As a substrate for several enzymes, including PLA2, PLC, and PKC, 4-(Trifluoromethyl)umbelliferyl phosphate disodium salt undergoes hydrolysis, leading to the formation of 4-trifluoromethylumbelliferone and phosphate. Subsequently, the 4-trifluoromethylumbelliferone can be further hydrolyzed, generating fluorescein as a byproduct, detectable through a fluorometer. By enabling such enzymatic processes and subsequent detection, 4-(Trifluoromethyl)umbelliferyl phosphate disodium salt empowers scientific investigations and facilitates valuable insights in various research domains.