Molecular structure of 4-Thiouracil, CAS Number: 591-28-6
4-Thiouracil (CAS 591-28-6)
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Alternate Names:
2-Hydroxy-4-mercaptopyrimidine
Application:
4-Thiouracil is a compound used in biosynthetic tagging in RNA analysis
CAS Number:
591-28-6
Purity:
≥97%
Molecular Weight:
128.15
Molecular Formula:
C4H4N2OS
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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4-Thiouracil is a nucleobase analogue of uracil in which the oxo group at C-2 is replaced by a thioxo group. 4-Thiouracil has been used in combination with spatially restricted uracil phosphoribosyltransferase for isolating RNA from cell types that are difficult to isolate by dissection or dissociation methods and should work in many organisms, including mammals and other vertebrates. Northwestern blotting techniques may also employ the use of 4-Thiouracil.
4-Thiouracil (CAS 591-28-6) References
- Synthesis and Characterization of Oxotechnetium(V) Mixed-Ligand Complexes Containing a Tridentate N-Substituted Bis(2-mercaptoethyl)amine and a Monodentate Thiol. | Pirmettis, IC., et al. 1996. Inorg Chem. 35: 1685-1691. PMID: 11666392
- Gas-phase reactivity of uracil, 2-thiouracil, 4-thiouracil, and 2,4-dithiouracil towards the Cu+ cation: a DFT study. | Lamsabhi, AM., et al. 2003. Chemphyschem. 4: 1011-6. PMID: 14562450
- RNA analysis by biosynthetic tagging using 4-thiouracil and uracil phosphoribosyltransferase. | Zeiner, GM., et al. 2008. Methods Mol Biol. 419: 135-46. PMID: 18369980
- Cell type-specific analysis of mRNA synthesis and decay in vivo with uracil phosphoribosyltransferase and 4-thiouracil. | Cleary, MD. 2008. Methods Enzymol. 448: 379-406. PMID: 19111186
- TU-tagging: cell type-specific RNA isolation from intact complex tissues. | Miller, MR., et al. 2009. Nat Methods. 6: 439-41. PMID: 19430475
- Photophysical and photochemical properties of 4-thiouracil: time-resolved IR spectroscopy and DFT studies. | Zou, X., et al. 2014. J Phys Chem B. 118: 5864-72. PMID: 24820207
- Toward Time-Resolved Analysis of RNA Metabolism in Archaea Using 4-Thiouracil. | Knüppel, R., et al. 2017. Front Microbiol. 8: 286. PMID: 28286499
- Time-Resolved Optical Pump-Resonant X-ray Probe Spectroscopy of 4-Thiouracil: A Simulation Study. | Nam, Y., et al. 2022. J Chem Theory Comput. 18: 3075-3088. PMID: 35476905
- Non-radioactive In Vivo Labeling of RNA with 4-Thiouracil. | Braun, C., et al. 2022. Methods Mol Biol. 2533: 199-213. PMID: 35796990
- Structure and tautomerism of the neutral and monoanionic forms of 4-thiouracil derivatives. | Psoda, A., et al. 1974. J Am Chem Soc. 96: 6832-9. PMID: 4436501
- Photoreaction of 4-thiouracil with cytosine. Relation to photoreactions in Escherichia coli transfer ribonucleic acids. | Bergstrom, DE. and Leonard, NJ. 1972. Biochemistry. 11: 1-9. PMID: 4550555
- Chemical transformation of 4-thiouracil nucleosides to uracil and cytosine counterparts. | Ziff, EB. and Fresco, JR. 1968. J Am Chem Soc. 90: 7338-42. PMID: 5725553
- Permanganate oxidation of 4-thiouracil derivatives. Isolation and properties of 1-substituted-2-pyrimidone-4-sulfonates. | Hayatsu, H. and Yano, M. 1969. Tetrahedron Lett. 755-8. PMID: 5794422
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
4-Thiouracil, 1 g | sc-256832 | 1 g | $103.00 |