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4-Nitrophenyl valerate is a chemical utilized primarily in enzymology research as a chromogenic and fluorogenic substrate. This ester compound is specifically designed for the assay of esterases and lipases, enzymes that catalyze the hydrolysis of ester bonds. When 4-Nitrophenyl valerate is hydrolyzed by these enzymes, it releases 4-nitrophenol, a compound that can be easily detected by its yellow color at alkaline pH or by fluorescence. This allows researchers to quantify enzyme activity by measuring the increase in absorbance or fluorescence. In studies of enzyme kinetics, 4-Nitrophenyl valerate is used to determine the specificity, rate, and inhibition of these enzymes. It is useful for understanding the role of lipolytic enzymes in various biological processes and can be used in the development and testing of enzyme inhibitors. Additionally, its application extends to the analysis of enzyme-substrate interactions and the screening of enzyme libraries for biotechnological applications.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
4-Nitrophenyl valerate, 1 g | sc-280458 | 1 g | $295.00 | |||
4-Nitrophenyl valerate, 5 g | sc-280458A | 5 g | $1100.00 |