4-Nitrophenyl bromoacetate (CAS 19199-82-7)

4-Nitrophenyl bromoacetate, known as 4-NPBA, is a widely used chemical compound in scientific research. It presents as a yellow crystalline solid, exhibiting solubility in water and ethanol. With its versatility as a reagent, 4-Nitrophenyl bromoacetate serves various purposes in laboratory applications, including synthesis, analysis, and biological research. In scientific research, 4-Nitrophenyl bromoacetate finds application across multiple domains. It is employed as a reagent for synthesizing diverse organic compounds, ranging from amines and esters to amides. Furthermore, it plays a vital role in protein and peptide analysis, facilitating the determination of molecular weight. Additionally, 4-Nitrophenyl bromoacetate contributes to the study of enzyme kinetics and enzyme inhibition. Regarding the mechanism of action, 4-Nitrophenyl bromoacetate acts as a competitive inhibitor of enzymes. By binding to the enzyme′s active site, it prevents the enzyme from interacting with its substrate. As a result, the enzyme is unable to catalyze the intended reaction, leading to inhibition of its activity.