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4-Nitrophenyl β-D-Galactofuranoside (CAS 100645-45-2)

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Alternate Names:
pNP-β-D-Galf
CAS Number:
100645-45-2
Molecular Weight:
301.25
Molecular Formula:
C12H15NO8
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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4-Nitrophenyl β-D-galactofuranoside is a synthetic compound used primarily as a chromogenic substrate in biochemical assays, particularly for detecting and measuring the activity of β-galactofuranosidases. These enzymes are part of the glycoside hydrolase family and specialize in cleaving the glycosidic bond in compounds that contain a galactofuranose moiety, a form of the sugar galactose where the ring structure is in a furanose (five-membered) configuration rather than the more common pyranose (six-membered) form. Upon enzymatic cleavage of this substrate, it releases 4-nitrophenol, a molecule that can be easily detected due to its yellow color when deprotonated under alkaline conditions. The release of 4-nitrophenol provides a direct and quantitative measure of enzyme activity. This is useful in various applications, including the characterization of enzymes from different biological sources, studying enzyme kinetics, and testing the effects of inhibitors or other modulatory factors on enzyme function.


4-Nitrophenyl β-D-Galactofuranoside (CAS 100645-45-2) References

  1. Immobilized 4-aminophenyl 1-thio-beta-D-galactofuranoside as a matrix for affinity purification of an exo-beta-D-galactofuranosidase.  |  Miletti, LC., et al. 1999. Carbohydr Res. 320: 176-82. PMID: 10573856
  2. Synthesis of 4-nitrophenyl beta-D-fucofuranoside and beta-D-fucofuranosyl-(1-->3)-D-mannopyranose: modified substrates for studies on catalytic requirements of beta-D-galactofuranosidase.  |  Chiocconi, A., et al. 2000. Carbohydr Res. 323: 7-13. PMID: 10782280
  3. An extracellular beta-galactofuranosidase from Aspergillus niger and its use as a tool for glycoconjugate analysis.  |  Wallis, GL., et al. 2001. Biochim Biophys Acta. 1525: 19-28. PMID: 11342249
  4. Synthesis of beta-D-galactofuranosyl nucleoside analogues. A new type of beta-D-galactofuranosidase inhibitor.  |  Marino, C., et al. 2001. Carbohydr Res. 333: 123-8. PMID: 11448672
  5. Influence of exo beta-D-galactofuranosidase inhibitors in cultures of Penicillium fellutanum and modifications in hyphal cell structure.  |  Mariño, K., et al. 2002. Carbohydr Res. 337: 891-7. PMID: 12007471
  6. Photoinduced electron transfer and chemical alpha-deoxygenation of D-galactono-1,4-lactone. Synthesis of 2-deoxy-D-lyxo-hexofuranosides.  |  Chiocconi, A., et al. 2002. Carbohydr Res. 337: 2119-26. PMID: 12433476
  7. Synthesis of 5-deoxy-beta-D-galactofuranosides as tools for the characterization of beta-D-galactofuranosidases.  |  Bordoni, A., et al. 2010. Bioorg Med Chem. 18: 5339-45. PMID: 20542704
  8. Detection of beta-galactofuranosidase production by Penicillium and Aspergillus species using 4-nitrophenyl beta-D-galactofuranoside.  |  Cousin, MA., et al. 1989. J Appl Bacteriol. 66: 311-7. PMID: 2502527
  9. Identification and Characterization of a Novel Galactofuranose-Specific β-D-Galactofuranosidase from Streptomyces Species.  |  Matsunaga, E., et al. 2015. PLoS One. 10: e0137230. PMID: 26340350
  10. Characterization of a PA14 domain-containing galactofuranose-specific β-d-galactofuranosidase from Streptomyces sp.  |  Matsunaga, E., et al. 2017. Biosci Biotechnol Biochem. 81: 1314-1319. PMID: 28317475
  11. Draft Genome Sequence of Streptomyces sp. JHA26, a Strain That Harbors a PA14 Domain Containing β-d-Galactofuranosidase.  |  Matsunaga, E., et al. 2017. Genome Announc. 5: PMID: 28408688
  12. Identification and characterization of β-d-galactofuranosidases from Aspergillus nidulans and Aspergillus fumigatus.  |  Matsunaga, E., et al. 2021. J Biosci Bioeng. 131: 1-7. PMID: 33011078
  13. 1-Thio-beta-D-galactofuranosides: synthesis and evaluation as beta-D-galactofuranosidase inhibitors.  |  Marino, C., et al. 1998. Glycobiology. 8: 901-4. PMID: 9675223

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

4-Nitrophenyl β-D-Galactofuranoside, 1 mg

sc-214263
1 mg
$152.00

4-Nitrophenyl β-D-Galactofuranoside, 10 mg

sc-214263A
10 mg
$183.00

4-Nitrophenyl β-D-Galactofuranoside, 25 mg

sc-214263B
25 mg
$328.00

4-Nitrophenyl β-D-Galactofuranoside, 50 mg

sc-214263C
50 mg
$464.00

4-Nitrophenyl β-D-Galactofuranoside, 100 mg

sc-214263D
100 mg
$693.00

4-Nitrophenyl β-D-Galactofuranoside, 200 mg

sc-214263E
200 mg
$1078.00