4-Nitrophenyl β-D-cellotrioside (CAS 106927-48-4)

4-Nitrophenyl β-D-cellotrioside (4-NPβCT) is an artificial carbohydrate compound derived from cellobiose, a disaccharide comprising two glucose molecules. This compound finds extensive use in various biochemical and physiological applications in scientific research. The key to its utility lies in its ability to imitate the structure of specific natural carbohydrates and its facile synthesis.In scientific research, 4-Nitrophenyl β-D-cellotrioside has proven valuable as a substrate for β-glucosidase, an enzyme that catalyzes the hydrolysis of β-glucosides.Its use extends to the investigation of cell-surface glycoproteins, exploring carbohydrate-protein interactions, studying the structure and function of certain enzymes, and examining the impact of carbohydrates and glycoproteins on cell signaling pathways.When 4-Nitrophenyl β-D-cellotrioside acts as a substrate for β-glucosidase, the enzyme cleaves the glycosidic bond between the two glucose molecules, liberating 4-nitrophenol and β-cellotriose. Subsequently, 4-nitrophenol can undergo further metabolism by enzymes like nitroreductase and nitrate reductase.