4-Nitrophenyl α-D-galactopyranoside (CAS 7493-95-0)
See product citations (1)
QUICK LINKS
4-Nitrophenyl α-D-galactopyranoside (4-NPG) is a synthetic compound consisting of a nitro group attached to a galactopyranoside. The applications of 4-Nitrophenyl α-D-galactopyranoside encompass diverse scientific research areas. It is frequently employed as a substrate for β-galactosidase, facilitating the detection of this enzyme in various cellular and tissue contexts. Moreover, it has been instrumental in investigating the structure and function of enzymes within the galactose metabolism pathway. The mechanism of action for 4-Nitrophenyl α-D-galactopyranoside is relatively straightforward. When present in an aqueous solution, the compound undergoes hydrolysis catalyzed by β-galactosidase. This reaction leads to the formation of galactose and 4-nitrophenol. The resulting galactose can subsequently undergo further metabolism facilitated by other enzymes within the galactose metabolism pathway.
4-Nitrophenyl α-D-galactopyranoside (CAS 7493-95-0) References
- Purification and characterization of alpha-galactosidase from a thermophilic fungus Thermomyces lanuginosus. | Puchart, V., et al. 2001. Biochim Biophys Acta. 1524: 27-37. PMID: 11078955
- Comparative study of new alpha-galactosidases in transglycosylation reactions. | Spangenberg, P., et al. 2000. Carbohydr Res. 329: 65-73. PMID: 11086687
- Enzymic alpha-galactosylation of a cyclic glucotetrasaccharide derived from alternan. | Biely, P., et al. 2001. Carbohydr Res. 332: 299-303. PMID: 11376609
- Glycosylation of internal sugar residues of oligosaccharides catalyzed by alpha-galactosidase from Aspergillus fumigatus. | Puchart, V. and Biely, P. 2005. Biochim Biophys Acta. 1726: 206-16. PMID: 16150549
- Direct sugar binding to LacY measured by resonance energy transfer. | Smirnova, IN., et al. 2006. Biochemistry. 45: 15279-87. PMID: 17176050
- Activity of Debaryomyces hansenii UFV-1 α-galactosidases against α-D-galactopyranoside derivatives. | Viana, PA., et al. 2011. Carbohydr Res. 346: 602-5. PMID: 21345419
- Stereochemical course of hydrolytic reaction catalyzed by alpha-galactosidase from cold adaptable marine bacterium of genus Pseudoalteromonas. | Bakunina, IY., et al. 2014. Front Chem. 2: 89. PMID: 25353020
- Characterization of an acidic α-galactosidase from hemp (Cannabis sativa L.) seeds and its application in removal of raffinose family oligosaccharides (RFOs). | Zhang, W., et al. 2018. Acta Biochim Pol. 65: 383-389. PMID: 30188963
- Overexpression and characterization of a novel GH4 galactosidase with β-galactosidase activity from Bacillus velezensis SW5. | Li, N., et al. 2021. J Dairy Sci. 104: 9465-9477. PMID: 34127264
- Asparagus racemosus and Geodorum densiflorum lectins induce apoptosis in cancer cells by altering proteins and genes expression. | Kabir, SR., et al. 2021. Int J Biol Macromol. 191: 646-656. PMID: 34582909
- Identification of dual active sites in Caenorhabditis elegans GANA-1 protein: an ortholog of the human α-GAL a and α-NAGA enzymes. | Cheong, CSY., et al. 2022. J Biomol Struct Dyn. 1-16. PMID: 35694994
- Determination of acid hydrolases in human platelets. | Dangelmaier, CA. and Holmsen, H. 1980. Anal Biochem. 104: 182-91. PMID: 6247939
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
4-Nitrophenyl α-D-galactopyranoside, 100 mg | sc-220978 | 100 mg | $36.00 | |||
4-Nitrophenyl α-D-galactopyranoside, 500 mg | sc-220978A | 500 mg | $95.00 |