4-Nitrophenyl 2-O-(α-L-Fucopyranosyl)-α-D-galactopyranoside (CAS 383417-46-7)

4-Nitrophenyl 2-O-(α-L-Fucopyranosyl)-α-D-galactopyranoside is a compound of interest in glycobiology research, particularly in studies involving carbohydrate recognition events and enzyme activity assays. Its mechanism of action revolves around its role as a substrate analog for enzymes involved in glycoside hydrolysis, specifically those that target α-L-fucosidases and α-D-galactosidases. Researchers utilize this compound as a chromogenic substrate to assess the activity of these enzymes, allowing for the quantification and characterization of their catalytic properties. Additionally, 4-Nitrophenyl 2-O-(α-L-Fucopyranosyl)-α-D-galactopyranoside serves as a probe in studies investigating carbohydrate-binding proteins, such as lectins, antibodies, and glycan-binding receptors. By exploiting its structural resemblance to natural glycoconjugates, scientists can explore carbohydrate-protein interactions, explain binding specificity, and decipher the biological functions of glycan recognition events. Furthermore, this compound finds application in the development of biosensors for detecting carbohydrate-active enzymes and glycan-binding proteins, contributing to advancements in glycobiology research and facilitating the study of glycan-mediated processes in various biological systems.