4-Nitrophenyl 2-O-(2,3,4,6-Tri-O-acetyl-α-D-mannopyranosyl)-α-D-mannopyranoside (CAS 26255-69-6)

4-Nitrophenyl 2-O-(2,3,4,6-Tri-O-acetyl-α-D-mannopyranosyl)-α-D-mannopyranoside is a crucial chemical tool widely employed in glycobiology research due to its specific mechanism of action and diverse applications. This compound serves as a substrate for glycosidase enzymes, particularly α-mannosidases, mimicking natural α-mannosides. The acetyl groups protect the hydroxyl groups of the sugar residues, enabling selective enzymatic hydrolysis. Upon enzymatic cleavage by α-mannosidases, the nitrophenyl moiety is released, generating a detectable colorimetric or fluorometric signal. This property is extensively utilized in enzymatic assays to study enzyme kinetics, substrate specificity, and inhibitor screening. Moreover, 4-Nitrophenyl 2-O-(2,3,4,6-Tri-O-acetyl-α-D-mannopyranosyl)-α-D-mannopyranoside finds applications in glycan biosynthesis studies, glycoconjugate synthesis, and the development of carbohydrate-based probes for biological imaging. Additionally, it contributes to the elucidation of glycan processing pathways and the exploration of glycosylation-related diseases. Overall, this chemical plays a pivotal role in advancing our understanding of glycosidase enzymes and their involvement in various biological processes, facilitating research in glycobiology, enzymology, and drug discovery.