4-Nitrophenyl 2-Benzoyl-4,6-O-benzylidene-α-D-mannopyranoside (CAS 94063-92-0)

4-Nitrophenyl 2-Benzoyl-4,6-O-benzylidene-α-D-mannopyranoside is a chemical compound extensively employed in biochemical research, particularly in the study of carbohydrate chemistry and enzymology. Its structure comprises a 4-nitrophenyl group attached to a benzoyl-substituted mannose residue, with a benzylidene moiety at the C-4 and C-6 positions of the sugar ring. This compound serves as a synthetic substrate for various glycosidases, enzymes responsible for catalyzing the hydrolysis of glycosidic bonds in carbohydrates. By utilizing 4-Nitrophenyl 2-Benzoyl-4,6-O-benzylidene-α-D-mannopyranoside in enzymatic assays, researchers can investigate the substrate specificity, kinetic parameters, and inhibition mechanisms of glycosidases. Furthermore, its benzoyl and benzylidene functionalities enable selective derivatization and immobilization onto solid supports, facilitating the development of glycosidase sensors and affinity chromatography matrices for the purification and characterization of glycosidases from complex biological samples. Moreover, this compound has been utilized as a model substrate to explain the molecular mechanisms underlying glycoside hydrolysis, providing insights into the structural determinants governing enzyme-substrate interactions and the catalytic mechanisms of glycosidases. Overall, 4-Nitrophenyl 2-Benzoyl-4,6-O-benzylidene-α-D-mannopyranoside plays a pivotal role in advancing our understanding of carbohydrate metabolism and enzymatic processes in biological systems.