4-Nitrophenyl 2,3,4-tri-O-acetyl-β-D-glucuronic Acid, Methyl Ester (CAS 18472-49-6)

4-Nitrophenyl 2,3,4-tri-O-acetyl-β-D-glucuronic Acid, Methyl Ester is a chemical compound commonly employed in biochemical research, particularly in studies related to glycosaminoglycan metabolism and enzyme kinetics. This compound serves as a substrate analog for glycosaminoglycan-degrading enzymes such as β-glucuronidases. By mimicking the structure of natural glycosaminoglycan substrates, it allows researchers to investigate the catalytic mechanisms and substrate specificity of these enzymes. In experimental setups, the enzymatic hydrolysis of 4-Nitrophenyl 2,3,4-tri-O-acetyl-β-D-glucuronic Acid, Methyl Ester can be monitored spectrophotometrically, enabling the determination of enzymatic kinetics parameters such as Michaelis-Menten constants and turnover rates. Furthermore, this compound is utilized in inhibitor screening assays to identify potential modulators of glycosaminoglycan-degrading enzymes, offering insights into strategies for conditions involving abnormal glycosaminoglycan metabolism. Additionally, it serves as a valuable tool in carbohydrate chemistry research, facilitating the synthesis and characterization of glycosaminoglycan analogs and derivatives. Overall, 4-Nitrophenyl 2,3,4-tri-O-acetyl-β-D-glucuronic Acid, Methyl Ester plays a crucial role in advancing our understanding of glycosaminoglycan metabolism and enzyme kinetics, with implications for both basic science and drug discovery efforts.