4-Methylumbelliferyl phosphate bis (cyclohexylammonium) salt (CAS 128218-53-1)

4-Methylumbelliferyl phosphate bis(cyclohexylammonium) salt, referred to as 4-MUP, serves as a fluorescent substrate of significant value in numerous biochemical and physiological experiments. Specifically, it functions as a phosphomonoesterase substrate, facilitating the measurement of phosphomonoesterase enzyme activity. The versatility of 4-Methylumbelliferyl phosphate bis(cyclohexylammonium) salt extends to its utilization in various laboratory experiments, including enzyme activity assessment, enzyme kinetics investigation, and enzyme inhibitor determination. Functionally, 4-Methylumbelliferyl phosphate bis(cyclohexylammonium) salt serves as a substrate for the hydrolysis performed by the phosphomonoesterase enzyme. This hydrolysis liberates 4-methylumbelliferone, a fluorescent molecule. The magnitude of fluorescence emitted by 4-methylumbelliferone is directly proportional to the enzyme quantity present. By measuring the fluorescence intensity of 4-methylumbelliferone, researchers can accurately determine the enzyme concentration.