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4-Methylumbelliferyl Oleate (CAS 18323-58-5)

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Application:
4-Methylumbelliferyl Oleate is a fluorogenic substrate for lipases
CAS Number:
18323-58-5
Molecular Weight:
440.61
Molecular Formula:
C28H40O4
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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4-Methylumbelliferyl Oleate is a substrate. When acted upon by lipases, 4-Methylumbelliferyl Oleate is hydrolyzed to release the fluorescent compound 4-Methylumbelliferone. This fluorescence can be measured and quantified. The mechanism of action involves the interaction of the lipase enzyme with the ester bond in 4-Methylumbelliferyl Oleate, leading to its cleavage and the subsequent release of 4-Methylumbelliferone. This process provides insights into the function and activity of lipases, contributing to a deeper understanding of lipid metabolism and related biochemical pathways. In experimental applications, 4-Methylumbelliferyl Oleate serves for investigating the role of lipases in various biological processes.


4-Methylumbelliferyl Oleate (CAS 18323-58-5) References

  1. Screening of lipase inhibitors from marine algae.  |  Bitou, N., et al. 1999. Lipids. 34: 441-5. PMID: 10380115
  2. Effect of dimethylsulfoxide on hydrolysis of lipase.  |  Tsuzuki, W., et al. 2001. Biosci Biotechnol Biochem. 65: 2078-82. PMID: 11676024
  3. Polar organic solvent added to an aqueous solution changes hydrolytic property of lipase.  |  Tsuzuki, W., et al. 2003. Biosci Biotechnol Biochem. 67: 1660-6. PMID: 12951497
  4. Pancreatic lipase inhibitory activity of taraxacum officinale in vitro and in vivo.  |  Zhang, J., et al. 2008. Nutr Res Pract. 2: 200-3. PMID: 20016719
  5. Hypolipidemic effect of Pleurotus eryngii extract in fat-loaded mice.  |  Mizutani, T., et al. 2010. J Nutr Sci Vitaminol (Tokyo). 56: 48-53. PMID: 20354346
  6. UHPLC/HR-ESI-MS/MS Profiling of Phenolics from Tunisian Lycium arabicum Boiss. Antioxidant and Anti-lipase Activities' Evaluation.  |  Affes, M., et al. 2017. Chem Biodivers. 14: PMID: 28613438
  7. Lipid metabolism in xanthomatous skin of hypercholesterolemic rabbits.  |  Armstrong, ML., et al. 1986. Am J Pathol. 125: 339-48. PMID: 2878618
  8. Inhibitory effects of Tiliacora triandra (Colebr.) Diels on cholesterol absorption.  |  Duangjai, A. and Saokaew, S. 2018. J Complement Integr Med. 16: PMID: 30312160
  9. Measuring Lipolytic Activity to Support Process Improvements to Manage Lipase-Mediated Polysorbate Degradation.  |  Jahn, M., et al. 2020. Pharm Res. 37: 118. PMID: 32495187
  10. Design, Synthesis, and Structure-Activity Relationship Study of Pyrazolones as Potent Inhibitors of Pancreatic Lipase.  |  Zhang, J., et al. 2021. ChemMedChem. 16: 1600-1604. PMID: 33527731
  11. Proanthocyanidins as the main pancreatic lipase inhibitors in chokeberry fruits.  |  Sosnowska, D., et al. 2022. Food Funct. 13: 5616-5625. PMID: 35506494
  12. Measurement of lipase activity of guinea pig peritoneal macrophages with 4-methylumbelliferyl-oleate.  |  Kiyotani, K., et al. 1983. Hiroshima J Med Sci. 32: 15-8. PMID: 6688069
  13. Characteristics of acid lipase and acid cholesteryl esterase activity in parenchymal and non-parenchymal rat liver cells.  |  Van Berkel, TJ., et al. 1980. Biochim Biophys Acta. 617: 446-57. PMID: 6768394
  14. Study of the hydrolysis of 4-methylumbelliferyl oleate by acid lipase and cholesteryl oleate by acid cholesteryl esterase in human leucocytes, fibroblasts and liver.  |  Koster, JF., et al. 1980. Biochim Biophys Acta. 618: 98-105. PMID: 6892888
  15. Purification and properties of human placental acid lipase.  |  Burton, BK. and Mueller, HW. 1980. Biochim Biophys Acta. 618: 449-60. PMID: 6893161

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

4-Methylumbelliferyl Oleate, 25 mg

sc-206915
25 mg
$99.00

4-Methylumbelliferyl Oleate, 100 mg

sc-206915A
100 mg
$310.00

We are experiencing high background with the 4-MUO that we have purchased from Sigma and are seeking an alternate source. Is the product you sell, sc-206915, prepared by you or is Sigma still the ultimate source? Thanks

Asked by: Diane P
Thank you for your question. This information is proprietary, please contact our Technical Service Department for further support.
Answered by: Technical Support
Date published: 2019-01-26

Do you have the 4-Methylumbelliferyl oleate auto-degradation data under different buffer or different pH?

Asked by: Mike Wang
Thank you for your question. We do not have this information available. If you need any further assistance, please contact our Technical Service department by phone at 1-800-457-3801 or by e-mail at scbt@scbt.com
Answered by: Technical Service
Date published: 2016-10-27
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Rated 5 out of 5 by from Various publications cite the use of 4Various publications cite the use of 4-Methylumbelliferyl Oleate as a suitable fluorogenic substrate for lipases. -SCBT Publication Review
Date published: 2015-07-04
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4-Methylumbelliferyl Oleate is rated 5.0 out of 5 by 1.
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