4-Methylumbelliferyl β-D-Galactopyranoside-6-sulfate Sodium Salt (CAS 206443-06-3)
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4-Methylumbelliferyl β-D-Galactopyranoside-6-sulfate sodium salt is a specialized compound utilized in biochemistry and enzymology research. It serves as a fluorogenic substrate for the enzyme β-galactosidase, where upon enzymatic cleavage, it releases a fluorescent product that can be quantitatively measured. This property is particularly useful in the study of enzyme kinetics, allowing researchers to track the reaction in real time and determine the enzyme′s activity under various conditions. Additionally, this compound is instrumental in the investigation of sulfatase activity, as the sulfate group provides specificity for certain types of sulfatases. The use of 4-Methylumbelliferyl β-D-Galactopyranoside-6-sulfate sodium salt in assays contributes to the understanding of genetic disorders related to deficiencies in these enzymes. In molecular biology, it aids in the screening of bacterial colonies for recombinant vectors that express β-galactosidase, a common reporter gene in cloning experiments.
4-Methylumbelliferyl β-D-Galactopyranoside-6-sulfate Sodium Salt (CAS 206443-06-3) References
- Effect of elongation factor 1alpha promoter and SUMF1 over in vitro expression of N-acetylgalactosamine-6-sulfate sulfatase. | Alméciga-Díaz, CJ., et al. 2009. Mol Biol Rep. 36: 1863-70. PMID: 18989752
- Enzyme replacement therapy for Morquio A: an active recombinant N-acetylgalactosamine-6-sulfate sulfatase produced in Escherichia coli BL21. | Rodríguez, A., et al. 2010. J Ind Microbiol Biotechnol. 37: 1193-201. PMID: 20582614
- Enzyme replacement therapy in newborn mucopolysaccharidosis IVA mice: early treatment rescues bone lesions? | Tomatsu, S., et al. 2015. Mol Genet Metab. 114: 195-202. PMID: 24953405
- Computational analysis of human N-acetylgalactosamine-6-sulfate sulfatase enzyme: an update in genotype-phenotype correlation for Morquio A. | Olarte-Avellaneda, S., et al. 2014. Mol Biol Rep. 41: 7073-88. PMID: 25287660
- Diagnosis of Morquio Syndrome in Dried Blood Spots Based on a New MRM-MS Assay. | Cozma, C., et al. 2015. PLoS One. 10: e0131228. PMID: 26147980
- Recombinant human N-acetylgalactosamine-6-sulfate sulfatase (GALNS) produced in the methylotrophic yeast Pichia pastoris. | Rodríguez-López, A., et al. 2016. Sci Rep. 6: 29329. PMID: 27378276
- Development of a fluorometric microtiter plate based enzyme assay for MPS IVA (Morquio type A) using dried blood spots. | Ullal, AJ., et al. 2014. Mol Genet Metab Rep. 1: 461-464. PMID: 27896123
- Diagnosing Mucopolysaccharidosis type IV a by the fluorometric assay of N-Acetylgalactosamine-6-sulfate sulfatase activity. | Shams, S., et al. 2017. J Diabetes Metab Disord. 16: 37. PMID: 28904929
- Characterization of Human Recombinant N-Acetylgalactosamine-6-Sulfate Sulfatase Produced in Pichia pastoris as Potential Enzyme for Mucopolysaccharidosis IVA Treatment. | Rodríguez-López, A., et al. 2019. J Pharm Sci. 108: 2534-2541. PMID: 30959056
- Identification of Ezetimibe and Pranlukast as Pharmacological Chaperones for the Treatment of the Rare Disease Mucopolysaccharidosis Type IVA. | Alméciga-Diaz, CJ., et al. 2019. J Med Chem. 62: 6175-6189. PMID: 31188588
- Treatment of skeletal and non-skeletal alterations of Mucopolysaccharidosis type IVA by AAV-mediated gene therapy. | Bertolin, J., et al. 2021. Nat Commun. 12: 5343. PMID: 34504088
- Activation of mitochondrial TRAP1 stimulates mitochondria-lysosome crosstalk and correction of lysosomal dysfunction. | Chen, FW., et al. 2022. iScience. 25: 104941. PMID: 36065186
- Association of N-acetylgalactosamine-6-sulfate sulfatase with the multienzyme lysosomal complex of beta-galactosidase, cathepsin A, and neuraminidase. Possible implication for intralysosomal catabolism of keratan sulfate. | Pshezhetsky, AV. and Potier, M. 1996. J Biol Chem. 271: 28359-65. PMID: 8910459
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
4-Methylumbelliferyl β-D-Galactopyranoside-6-sulfate Sodium Salt, 10 mg | sc-220946 | 10 mg | $398.00 |