Date published: 2026-2-17

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4-Methylumbelliferyl β-D-cellobioside (CAS 72626-61-0)

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Application:
4-Methylumbelliferyl β-D-cellobioside is a substrate used to measure the activity of (1,4)-β-Glucanase
CAS Number:
72626-61-0
Purity:
≥98%
Molecular Weight:
500.45
Molecular Formula:
C22H28O13
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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4-Methylumbelliferyl β-D-cellobioside can be used in Cel D Fusion screenings and activity staining in polyacrylamide gels. 4-Methylumbelliferyl β-D-cellobioside is a sugar-containing molecule known as a cellobioside, which consists of a glucose molecule linked to a sugar alcohol called cellobiose. The presence of the 4-methylumbelliferyl group confers hydrophobic properties to the molecule, making it more soluble in organic solvents. Scientists have utilized this compound in studies aimed at investigating how different enzymes impact carbohydrate metabolism. Moreover, 4-Methylumbelliferyl β-D-cellobioside has been useful in understanding the mechanisms of action of various enzymes, such as glycosidases and glycosyltransferases.


4-Methylumbelliferyl β-D-cellobioside (CAS 72626-61-0) References

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  2. Identification and expression of cellobiohydrolase (CBHI) gene from an endophytic fungus, Fusicoccum sp. (BCC4124) in Pichia pastoris.  |  Kanokratana, P., et al. 2008. Protein Expr Purif. 58: 148-53. PMID: 17964183
  3. Screening and characterization of an enzyme with beta-glucosidase activity from environmental DNA.  |  Kim, SJ., et al. 2007. J Microbiol Biotechnol. 17: 905-12. PMID: 18050907
  4. Strategy for screening metagenomic resources for exocellulase activity using a robotic, high-throughput screening system.  |  Ko, KC., et al. 2013. J Microbiol Methods. 94: 311-6. PMID: 23892060
  5. Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex.  |  Dojnov, B., et al. 2015. Electrophoresis. 36: 1724-7. PMID: 25873475
  6. Isolation of aerobic cultivable cellulolytic bacteria from different regions of the gastrointestinal tract of giant land snail Achatina fulica.  |  Pinheiro, GL., et al. 2015. Front Microbiol. 6: 860. PMID: 26347735
  7. Continuous photometric determination of endo-1,4-beta-D-glucanase (cellulase) activity using 4-methylumbelliferyl-beta-D-cellobioside as a substrate.  |  Chernoglazov, VM., et al. 1989. Anal Biochem. 179: 186-9. PMID: 2757193
  8. Purification and characterization of an extracellular β-glucosidase from Sporothrix schenckii.  |  Hernández-Guzmán, A., et al. 2016. FEBS Open Bio. 6: 1067-1077. PMID: 27833847
  9. Cloning, Production and Characterization of a Glycoside Hydrolase Family 7 Enzyme from the Gut Microbiota of the Termite Coptotermes curvignathus.  |  Woon, JS., et al. 2017. Mol Biotechnol. 59: 271-283. PMID: 28573450
  10. Cellobiohydrolase B of Aspergillus niger over-expressed in Pichia pastoris stimulates hydrolysis of oil palm empty fruit bunches.  |  Woon, JS., et al. 2017. PeerJ. 5: e3909. PMID: 29038760
  11. Effects of Oligosaccharides Isolated From Pinewood Hot Water Pre-hydrolyzates on Recombinant Cellulases.  |  Fang, H., et al. 2018. Front Bioeng Biotechnol. 6: 55. PMID: 29868572
  12. Rapid and Quantitative CELD Assay to Measure the Specificity of Transcription Factor-DNA-Binding Interactions and Identify cis-Elements.  |  Kalaipandian, S. and Xue, GP. 2018. Methods Mol Biol. 1830: 307-324. PMID: 30043378
  13. Molecular cloning of multiple xylanase genes from Pseudomonas fluorescens subsp. cellulosa.  |  Gilbert, HJ., et al. 1988. J Gen Microbiol. 134: 3239-47. PMID: 3151992
  14. Millipede gut-derived microbes as a potential source of cellulolytic enzymes.  |  Koubová, A., et al. 2023. World J Microbiol Biotechnol. 39: 169. PMID: 37186294
  15. The bifunctional enzyme chitosanase-cellulase produced by the gram-negative microorganism Myxobacter sp. AL-1 is highly similar to Bacillus subtilis endoglucanases.  |  Pedraza-Reyes, M. and Gutiérrez-Corona, F. 1997. Arch Microbiol. 168: 321-7. PMID: 9297470

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

4-Methylumbelliferyl β-D-cellobioside, 100 mg

sc-220943
100 mg
$121.00

4-Methylumbelliferyl β-D-cellobioside, 500 mg

sc-220943A
500 mg
$407.00

4-Methylumbelliferyl β-D-cellobioside, 2 g

sc-220943B
2 g
$962.00

4-Methylumbelliferyl β-D-cellobioside, 5 g

sc-220943C
5 g
$1413.00