4-Methylumbelliferyl 2-Amino-2-deoxy-α-D-glucopyranoside (CAS 137687-00-4)

4-Methylumbelliferyl 2-Amino-2-deoxy-α-D-glucopyranoside is a fluorogenic substrate commonly used in enzymatic assays to detect and quantify the activity of β-glucosidase enzymes. Its mechanism of action involves the enzymatic hydrolysis of the glycosidic bond by β-glucosidase, resulting in the release of the fluorescent molecule 4-methylumbelliferone (4-MU). This compound serves as a sensitive and specific probe for β-glucosidase activity, as the fluorescence of 4-MU can be easily detected and quantified using fluorescence spectroscopy or fluorometric assays. Researchers utilize 4-Methylumbelliferyl 2-Amino-2-deoxy-α-D-glucopyranoside in various biological and biochemical studies to investigate the role of β-glucosidase enzymes in carbohydrate metabolism, cellular signaling, and disease pathogenesis. Additionally, this compound finds applications in high-throughput screening assays for identifying potential inhibitors or activators of β-glucosidase activity, facilitating drug discovery efforts targeting β-glucosidase-related diseases such as Gaucher′s disease and certain cancers. Through its specific mechanism of action and versatile research applications, 4-Methylumbelliferyl 2-Amino-2-deoxy-α-D-glucopyranoside continues to be a valuable tool in elucidating the biochemical pathways and molecular mechanisms involving β-glucosidase enzymes.