4-Hydroxynonenal is an aldehydic product of ω-6-unsaturated fatty acid peroxidation. It is considered a lipid peroxidation specific marker. It has been found to induce differentiation and inhibit proliferation of HL-60 human leukemic cells. It has also been found to induce murine alveolar macrophage cell death. It has been shown to inhibit State 3 respiration, causing a transient cytosolic Ca2+ increase. It has also been shown to irreversibly inhibit Na+/K+-ATPase activity. 4-Hydroxynonenal is an inhibitor of JNK and an activator of ANKTM1.
If an organic solvent-free aqueous solution is needed, it can be prepared by evaporating the ethanol under a gentle stream of nitrogen and directly dissolving it in an aqueous buffer. Aqueous solutions should be kept at neutral pH, since HNE undergoes polymerization in basic and acidic conditions. The solubility of HNE in PBS (pH 7.2) is ~1 mg/ml. Store aqueous solutions of HNE on ice and use within 6 hours of preparation. Avoid freeze/thaw cycles.
a solution in ethanol
Soluble in 100% ethanol, DMSO (~50 mg/ml), DMF (~50 mg/ml), and PBS (pH 7.2) (~1 mg/ml).
Dear Sir, I am interested in testing if my GST has activity against 4hydroxynonenal and 4 Hydroxydecenal. So can find these products in Brazil? Do you have any protocols for this experiment? How do I handle these substrates?
Asked by: CharlesJr
Thank you for your question. Please contact one of our authorized distributors for information about current product availability and shipping lead times. We do not provide any protocols for your specific research experiment. We recommend you consult the primary literature. Please refer to the Safety Data Sheet (SDS) online for handling of this reagent.
Answered by: Technical Support
Date published: 2019-07-13
can this be run through ELIZA ?
Asked by: Vozah
Thank you for your question. We do not have experience using this compound for an ELISA. It is possible. This research grade compound is supplied for in vitro and analytical testing. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-06-20
What is the concentration of this product?
Asked by: two2igm05
Thank you for your question. 4-Hydroxynonenal, sc-202019, is supplied in 10 mg/ml.
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