4-Hydroxynonenal CAS: 75899-68-2
MF: C9H16O2
MW: 156.22

4-Hydroxynonenal (CAS 75899-68-2)

4-Hydroxynonenal | CAS 75899-68-2 is rated 5.0 out of 5 by 1.
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Alternate Names: (±)-4-hydroxy-2E-nonenal; 4-HNE
Application: 4-Hydroxynonenal is an irreversible inhibitor of Na+/K+-ATPase activity
CAS Number: 75899-68-2
Purity: ≥98%
Molecular Weight: 156.22
Molecular Formula: C9H16O2
Supplemental Information: This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).

4-Hydroxynonenal is an aldehydic product of ω-6-unsaturated fatty acid peroxidation. It is considered a lipid peroxidation specific marker. It has been found to induce differentiation and inhibit proliferation of HL-60 human leukemic cells. It has also been found to induce murine alveolar macrophage cell death. It has been shown to inhibit State 3 respiration, causing a transient cytosolic Ca2+ increase. It has also been shown to irreversibly inhibit Na+/K+-ATPase activity. 4-Hydroxynonenal is an inhibitor of JNK and an activator of ANKTM1.


1. Esterbauer, H., et al. 1991. Free Radic. Biol. Med. 11: 81-128. PMID: 1937131
2. Carini, R., et al. 1996. Biochem. Biophys. Res. Commun. 218: 772-776. PMID: 8579589
3. Siems, W.G., et al. 1996. Free Radic. Biol. Med. 20: 215-223. PMID: 8746442
4. Ullrich, O., et al. 1996. Free Radic. Res. 24: 421-427. PMID: 8804985
5. Li, L., et al. 1996. Toxicol. Appl. Pharmacol. 139: 135-143. PMID: 8685896
6. Soh, Y., et al. 2000. Mol. Pharmacol. 58: 535-541. PMID: 10953046

Usage :
If an organic solvent-free aqueous solution is needed, it can be prepared by evaporating the ethanol under a gentle stream of nitrogen and directly dissolving it in an aqueous buffer. Aqueous solutions should be kept at neutral pH, since HNE undergoes polymerization in basic and acidic conditions. The solubility of HNE in PBS (pH 7.2) is ~1 mg/ml. Store aqueous solutions of HNE on ice and use within 6 hours of preparation. Avoid freeze/thaw cycles.
Formulation :
a solution in ethanol
Physical State :
Solubility :
Soluble in 100% ethanol, DMSO (~50 mg/ml), DMF (~50 mg/ml), and PBS (pH 7.2) (~1 mg/ml).
Storage :
Store at -80° C
Boiling Point :
78° C
Refractive Index :
n20D 1.46 (Predicted)
IC50 :
Na+, K+-ATPase activity : IC50 = 120 µM; Transient receptor potential cation channel subfamily A member 1: IC50 = 19.9 µM (rat)
pK Values :
pKa: 13.31 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
PubChem CID :
MDL Number :

Download SDS (MSDS)

Certificate of Analysis

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4-Hydroxynonenal (CAS 75899-68-2)  Product Citations

See how others have used 4-Hydroxynonenal (CAS 75899-68-2). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 16 total

PMID: # 31315052  Suzuki, T.|Muramatsu, A.|Saito, R.|Iso, T.|Shibata, T.|Kuwata, K.|Kawaguchi, SI.|Iwawaki, T.|Adachi, S.|Suda, H.|Morita, M.|Uchida, K.|Baird, L.|Yamamoto, M.| et al. 2019. Cell Rep. 28: 746-758.e4.

PMID: # 27697860  Iso, T. et al. 2016. Mol. Cell. Biol. 36: 3100-3112.

PMID: # 26608809  Valencia, AP. et al. 2016. Experimental gerontology. 73: 14-22.

PMID: # 25762099  Moraes, LH. et al. 2015. Biological trace element research. 167: 115-20.

PMID: # 26045757  Zhang, J. et al. 2015. International journal of clinical and experimental pathology. 8: 2515-24.

PMID: # 26527616  2015. Mol. Cell. Biol. 36: 271-84.

PMID: # 24258150  Aoi, J. et al. 2014. Molecular cancer research : MCR. 12: 239-49.

PMID: # 22989377  Lanceta, L. et al. 2013. The Biochemical journal. 449: 189-94.

PMID: # 22362175  Jeon, BT. et al. 2012. Diabetes. 61: 1444-54.

PMID: # 23115123  Cowley, PM. et al. 2012. American journal of physiology. Regulatory, integrative and comparative physiology. 303: R1251-60.

Citations 1 to 10 of 16 total

Dear Sir, I am interested in testing if my GST has activity against 4hydroxynonenal and 4 Hydroxydecenal. So can find these products in Brazil? Do you have any protocols for this experiment? How do I handle these substrates?

Asked by: CharlesJr
Thank you for your question. Please contact one of our authorized distributors for information about current product availability and shipping lead times. We do not provide any protocols for your specific research experiment. We recommend you consult the primary literature. Please refer to the Safety Data Sheet (SDS) online for handling of this reagent.
Answered by: Technical Support
Date published: 2019-07-13

can this be run through ELIZA ?

Asked by: Vozah
Thank you for your question. We do not have experience using this compound for an ELISA. It is possible. This research grade compound is supplied for in vitro and analytical testing. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-06-20

What is the concentration of this product?

Asked by: two2igm05
Thank you for your question. 4-Hydroxynonenal, sc-202019, is supplied in 10 mg/ml.
Answered by: Chemical Support 4
Date published: 2018-03-26
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Rated 5 out of 5 by from Used for inducing cell death Used for inducing cell death, human cell line A549
Date published: 2015-04-09
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