3′,5′-Di-O-acetyl O6-Benzyl-2′-deoxyguanosine (CAS 144640-75-5)

3′,5′-Di-O-acetyl O6-Benzyl-2′-deoxyguanosine is a modified nucleoside derivative, crucial in molecular biology research for studying DNA repair and mutagenesis processes. Its unique structure allows for incorporation into DNA during replication, providing a model to study how cells recognize and repair altered nucleotides. The benzyl group at the O6 position mimics DNA adducts, offering insights into base pairing anomalies and their effect on polymerase fidelity. The acetyl modifications at the 3′ and 5′ positions enhance cellular uptake and stability, making it a valuable tool in research focused on nucleotide excision repair mechanisms and mismatch repair pathways. Studies employing this compound have explaind the role of specialized glycosylases and endonucleases in identifying and excising damaged bases, advancing the understanding of genome integrity maintenance. Moreover, it has been used in various high-throughput screenings to identify proteins and small molecules interacting with damaged nucleotides. Overall, it serves as a potent chemical probe, enabling researchers to dissect complex cellular pathways and better understand the fundamental mechanisms underpinning DNA repair fidelity.