Date published: 2025-12-13

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3-Oxo-3H-benzo[f]chromene-2-carboxylic acid (CAS 4361-00-6)

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CAS Number:
4361-00-6
Molecular Weight:
240.21
Molecular Formula:
C14H8O4
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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C-12 NBD Ceramide is a long-chain fluorescent ceramide analog that can be used for the measurement of alkaline and neutral ceramidase activity. It is hydrolyzed efficiently by alkaline ceramidase from P. aeruginosa and by neutral ceramidase from mouse liver. In addition, alkaline and neutral ceramidases from a variety of mammalian cell lines also hydrolyze C-12 NBD Ceramide. Acid ceramidase does not appear to utilize C-12 NBD Ceramide as a substrate. The possibility exists for C-12 NBD Ceramide to also be applied to the measurement of ceramide kinase and/or sphingomyelin synthase activity. Ceramidase catalyzes the hydrolysis of the N-acyl linkage between the fatty acid and sphingosine base in ceramide to produce sphingosine and a free fatty acid. Three isoforms of ceramidases (acid, neutral, and alkaline) have been characterized based on differences in their catalytic pH optimum.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

3-Oxo-3H-benzo[f]chromene-2-carboxylic acid, 500 mg

sc-313483
500 mg
$96.00