Date published: 2026-4-1

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3-Nitrophenylhydrazine hydrochloride (CAS 636-95-3)

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CAS Number:
636-95-3
Molecular Weight:
189.60
Molecular Formula:
C6H8ClN3O2
Supplemental Information:
This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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3-Nitrophenylhydrazine hydrochloride (3-NPH) is an organic compound which is used in many scientific experiments and research. It is a popular reagent used in various biochemical and physiological experiments due to its high reactivity. Specifically, 3-Nitrophenylhydrazine hydrochloride is employed in the analysis of carbohydrates, proteins, enzymes, and hormones. It is also used in the synthesis of various compounds such as 3-nitrophenylhydrazines, 3-nitrophenylhydrazones, and 3-nitro-4-hydroxybenzoic acids.


3-Nitrophenylhydrazine hydrochloride (CAS 636-95-3) References

  1. N,N'-Bis(2-chloronicotinoyl)-N-(3-nitrophenyl)hydrazine monohydrate: complex sheets built from O-H..N, N-H..O and C-H..O hydrogen bonds.  |  Wardell, SM., et al. 2006. Acta Crystallogr C. 62: o170-2. PMID: 16598135
  2. Quantification of 13C pyruvate and 13C lactate in dog blood by reversed-phase liquid chromatography-electrospray ionization mass spectrometry after derivatization with 3-nitrophenylhydrazine.  |  Uran, S., et al. 2007. J Pharm Biomed Anal. 44: 947-54. PMID: 17482415
  3. Synthesis and exploration of novel curcumin analogues as anti-malarial agents.  |  Mishra, S., et al. 2008. Bioorg Med Chem. 16: 2894-902. PMID: 18194869
  4. Determination of residual 4-nitrobenzaldehyde in chloramphenicol and its pharmaceutical formulation by HPLC with UV/Vis detection after derivatization with 3-nitrophenylhydrazine.  |  Luo, L., et al. 2018. J Pharm Biomed Anal. 156: 307-312. PMID: 29730340
  5. Rapid, High-Throughput Quantitation of Odor-Active 2-Acetyl Azaheterocycles in Food Products by UHPLC-MS/MS.  |  Bösl, M., et al. 2021. J Agric Food Chem. 69: 1405-1412. PMID: 33470094
  6. Serum metabolic signatures of subclinical atherosclerosis in patients with type 2 diabetes mellitus: a preliminary study.  |  Su, J., et al. 2021. Acta Diabetol. 58: 1217-1224. PMID: 33871690
  7. Method validation of gamma-Hydroxybutyric acid detection upon Herpes Simplex Virus-Type 1 infection using LC-MRM-MS with 3-nitrophenylhydrazine derivatization.  |  Osinaga, FO., et al. 2022. J Pharm Biomed Anal. 210: 114547. PMID: 35042145
  8. Optimised Method for Short-Chain Fatty Acid Profiling of Bovine Milk and Serum.  |  Li, C., et al. 2022. Molecules. 27: PMID: 35056750
  9. The usefulness of measuring n-butyric acid concentration as a new indicator of blood decomposition in forensic autopsy.  |  Matoba, K., et al. 2022. Leg Med (Tokyo). 57: 102071. PMID: 35461038
  10. Improved quantitation of short-chain carboxylic acids in human biofluids using 3-nitrophenylhydrazine derivatization and liquid chromatography with tandem mass spectrometry (LC-MS/MS).  |  Valdivia-Garcia, MA., et al. 2022. J Pharm Biomed Anal. 221: 115060. PMID: 36166933
  11. Structural elucidation of 3-nitrophenylhydrazine derivatives of tricarboxylic acid cycle acids and optimization of their fragmentation to boost sensitivity in liquid chromatography-mass spectrometry.  |  Hodek, O., et al. 2023. J Chromatogr B Analyt Technol Biomed Life Sci. 1222: 123719. PMID: 37060816
  12. Stable isotope labelling-flow injection analysis-mass spectrometry for rapid and high throughput quantitative analysis of 5-hydroxymethylfurfural in drinks  |  ZH Wang, JX Zhang, M Gao, WQ Cui, L Xu, X Zhu, JJ Li… - Food Control, 2021 - Elsevier. December 2021,. Food Control. Volume 130,: 108386.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

3-Nitrophenylhydrazine hydrochloride, 5 g

sc-231886
5 g
$70.00

3-Nitrophenylhydrazine hydrochloride, 25 g

sc-231886A
25 g
$204.00