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3-Indoxyl-β-D-glucopyranoside hydrate is a chromogenic substrate used in various research applications, particularly within the field of enzymology. It is employed to assay the activity of β-glucosidase enzymes, which catalyze the hydrolysis of glycosidic bonds in β-glucosides. Upon enzymatic cleavage, this substrate yields an indigo-blue product that is easily quantifiable, allowing researchers to monitor enzyme activity in real-time. This characteristic makes it valuable in environmental microbiology for detecting and quantifying β-glucosidase-producing microorganisms in soil samples, which is important for understanding nutrient cycling and soil health. Additionally, its use extends to molecular genetics, where it aids in the identification of gene expression markers in β-glucosidase reporter assays. The hydrate form of this compound ensures enhanced solubility and stability, making it suitable for use in various assay conditions.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
3-Indoxyl-β-D-glucopyranoside hydrate, 25 mg | sc-473538 | 25 mg | $141.00 |