Date published: 2025-9-5

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3,5-Dinitrosalicylic acid (CAS 609-99-4)

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Application:
3,5-Dinitrosalicylic acid is a salicylic acid derivative used in colorimeteric determination of reducing sugars
CAS Number:
609-99-4
Purity:
≥98%
Molecular Weight:
228.12
Molecular Formula:
C7H4N2O7
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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3,5-Dinitrosalicylic acid is a dinitrated derivative of salicylic acid. 3,5-Dinitrosalicylic acid is used in preparing DISAL glycosyl donors. Proton-transfer compounds composed of 3,5-Dinitrosalicylic acid/Strychnine and 3,5-Dinitrosalicylic acid/benzidine have also been described.


3,5-Dinitrosalicylic acid (CAS 609-99-4) References

  1. Efficient synthesis of glycosylated phenazine natural products and analogs with DISAL (methyl 3,5-dinitrosalicylate) glycosyl donors.  |  Laursen, JB., et al. 2003. Org Biomol Chem. 1: 3147-53. PMID: 14527145
  2. The proton-transfer compounds of strychnine with achiral salicylic acids: strychninium 3,5-dinitrosalicylate and the strychninium 5-nitrosalicylate bis(5-nitrosalicylic acid) adduct.  |  Smith, G., et al. 2005. Acta Crystallogr C. 61: o464-8. PMID: 15997084
  3. Mixed dicationic and monocationic benzidine species in the proton-transfer compound of benzidine with 3,5-dinitrosalicylic acid.  |  Smith, G., et al. 2006. Acta Crystallogr C. 62: o402-4. PMID: 16823214
  4. Anhydrous 1:1 proton-transfer compounds of isonipecotamide with picric acid and 3,5-dinitrosalicylic acid: 4-carbamoylpiperidinium 2,4,6-trinitrophenolate and two polymorphs of 4-carbamoylpiperidinium 2-carboxy-4,6-dinitrophenolate.  |  Smith, G. and Wermuth, UD. 2010. Acta Crystallogr C. 66: o609-13. PMID: 21123895
  5. Study on conformational stability, molecular structure, vibrational spectra, NBO, TD-DFT, HOMO and LUMO analysis of 3,5-dinitrosalicylic acid by DFT techniques.  |  Sebastian, S., et al. 2015. Spectrochim Acta A Mol Biomol Spectrosc. 136 Pt B: 1107-18. PMID: 25459508
  6. Pitfalls in the 3, 5-dinitrosalicylic acid (DNS) assay for the reducing sugars: Interference of furfural and 5-hydroxymethylfurfural.  |  Deshavath, NN., et al. 2020. Int J Biol Macromol. 156: 180-185. PMID: 32289426
  7. 3,5-Dinitrosalicylic Acid Adsorption Using Granulated and Powdered Activated Carbons.  |  Hernández, JA., et al. 2021. Molecules. 26: PMID: 34834010
  8. Conjugated hypercrosslinked polymers imprinted with 3,5-dinitrosalicylic acid for the fluorescent determination of α-amylase activity.  |  Yan, RY., et al. 2023. Spectrochim Acta A Mol Biomol Spectrosc. 291: 122383. PMID: 36682253
  9. Measuring Enzyme Kinetics of Glycoside Hydrolases Using the 3,5-Dinitrosalicylic Acid Assay.  |  Li, H. and McKee, LS. 2023. Methods Mol Biol. 2657: 15-25. PMID: 37149520
  10. Chromogenic Assay Is More Efficient in Identifying α-Amylase Inhibitory Properties of Anthocyanin-Rich Samples When Compared to the 3,5-Dinitrosalicylic Acid (DNS) Assay.  |  Zulfiqar, S., et al. 2023. Molecules. 28: PMID: 37687228

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

3,5-Dinitrosalicylic acid, 10 g

sc-214181
10 g
$33.00

3,5-Dinitrosalicylic acid, 25 g

sc-214181A
25 g
$53.00

3,5-Dinitrosalicylic acid, 100 g

sc-214181B
100 g
$109.00

3,5-Dinitrosalicylic acid, 500 g

sc-214181C
500 g
$425.00