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3,5-Dinitrobenzoyl chloride (CAS 99-33-2)

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CAS Number:
99-33-2
Molecular Weight:
230.56
Molecular Formula:
C7H3ClN2O5
Supplemental Information:
This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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3,5-Dinitrobenzoyl chloride is a reagent extensively used in analytical chemistry for the derivatization of alcohols and amines, facilitating their identification and quantification by improving their detectability in spectroscopic methods. Its role in the synthesis of dinitrophenyl (DNP) derivatives is particularly valued because these derivatives are significantly more chromophoric and can be easily detected even at low concentrations. Researchers also leverage this compound in the field of organic synthesis to introduce the dinitrobenzoyl protecting group, which can be selectively removed at a later stage of synthesis. This versatility makes it a staple in the preparation of complex organic molecules where stepwise functionalization is required. Furthermore, the compound is employed in the study of reaction mechanisms where its reactivity with different nucleophiles is monitored, offering insights into the steric and electronic effects within molecular structures.


3,5-Dinitrobenzoyl chloride (CAS 99-33-2) References

  1. A new method for the determination of serum creatinine based on reaction with 3.5-dinitrobenzoyl chloride in an organic medium.  |  Parekh, AC., et al. 1976. Clin Chim Acta. 73: 221-31. PMID: 1000844
  2. Liquid chromatographic determination of biogenic amines in fermented foods after derivatization with 3,5-dinitrobenzoyl chloride.  |  Kirschbaum, J., et al. 2000. J Chromatogr A. 881: 517-30. PMID: 10905732
  3. Sensitive determination of methylenedioxylated amphetamines by liquid chromatography.  |  Herráez-Hernández, R., et al. 2001. Analyst. 126: 581-6. PMID: 11394297
  4. A new method for the determination of 2,2'-dichloro-4,4'-methylenedianiline in workplace air samples by HPLC-DAD.  |  Jeżewska, A. and Buszewski, B. 2011. Toxicol Mech Methods. 21: 554-60. PMID: 21473712
  5. Homogeneous acylation of xylan with 3,5-dinitrobenzoyl in ionic liquid and the adsorption property.  |  Kong, W., et al. 2015. Carbohydr Polym. 128: 105-11. PMID: 26005145
  6. Isolation of Nicotinic Acid (Vitamin B3) and N-Propylamine after Myosmine Peroxidation.  |  Zwickenpflug, W., et al. 2016. J Agric Food Chem. 64: 178-84. PMID: 26673015
  7. Chiral separation on various modified amino alcohol-derived HPLC chiral stationary phases.  |  Yu, J., et al. 2016. Chirality. 28: 276-81. PMID: 26871459
  8. HPLC analysis of polyamines and their acetylated derivatives in the picomole range using benzoyl chloride and 3,5-dinitrobenzoyl chloride as derivatizing agent.  |  Wongyai, S., et al. 1988. Biomed Chromatogr. 2: 254-7. PMID: 3233395
  9. Chromatographic separation of the diastereomers of a dihydropyridine-type calcium channel antagonist as the bis-3,5-dinitrobenzoates.  |  Kern, JR., et al. 1988. J Chromatogr. 457: 309-16. PMID: 3243886
  10. Reactivity of creatinine with alkaline 3,5-dinitrobenzoate: a new fluorescent kidney function test.  |  Blass, KG. 1995. Clin Biochem. 28: 107-11. PMID: 7628067
  11. High-performance liquid chromatographic determination of taurine and hypotaurine using 3,5-dinitrobenzoyl chloride as derivatizing reagent.  |  Masuoka, N., et al. 1994. J Chromatogr B Biomed Appl. 660: 31-5. PMID: 7858721
  12. Bombesin stimulates distinct time-dependent changes in the sn-1,2-diradylglycerol molecular species profile from Swiss 3T3 fibroblasts as analysed by 3,5-dinitrobenzoyl derivatization and h.p.l.c. separation.  |  Pettitt, TR. and Wakelam, MJ. 1993. Biochem J. 289 (Pt 2): 487-95. PMID: 8424792
  13. Liquid chromatographic analysis of amphetamine and related compounds in urine using solid-phase extraction and 3,5-dinitrobenzoyl chloride for derivatization.  |  Herráez-Hernández, R., et al. 1997. J Chromatogr Sci. 35: 169-75. PMID: 9100339

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

3,5-Dinitrobenzoyl chloride, 25 g

sc-252064
25 g
$38.00

3,5-Dinitrobenzoyl chloride, 100 g

sc-252064A
100 g
$102.00